In specific genetic backgrounds, a mutation in the tuh-3 gene results in the homeotic transformation of head structures to either leg disc derivatives or structures normally found in the extreme posterior end of wild-type animals. The origins of the homeotic structures were mapped to defined positions in the eye-antennal imaginal disc by transplanting abnormal regions of discs isolated from tuh-3 mutants into host mwh;e4 larvae. These metamorphosed implants were removed and differentiated structures were identified. Of 211 successfully recovered implants, 157 gave rise to homeotic tissue: abdominal tergite, male or female external genitalia and/or leg tissue. Transformations to abdominal tergite occurred primarily in cells taken from the eye region of the compound disc. Male and female genitalia arose most often in implants taken from the antennal portion of the disc, although some tissue taken from the lateral region of the eye disc also gave rise to external genitalia. Leg structures came exclusively from implants from the antennal region of the imaginal disc. These results suggest that cells from within specific regions of the eye-antennal compound disc are constrained in their developmental potential. An obvious constraint observed with this mutation is a dorsal/ventral one: Cells from the eye disc, a dorsal structure, primarily gave rise to other dorsal structures, abdominal tergite tissue. Cells from the antennal disc, a ventrally derived structure, primarily gave rise to other ventral structures including genital tissue and distal leg.
Fate map of the eye-antennal imaginal disc of the tumorous-head mutant of Drosophila melanogaster
D.J. Andrew; Fate map of the eye-antennal imaginal disc of the tumorous-head mutant of Drosophila melanogaster. Development 1 June 1987; 100 (2): 261–269. doi: https://doi.org/10.1242/dev.100.2.261
Download citation file:
Advertisement
Cited by
Development Journal Meeting 2022: From Stem Cells to Human Development
-JournalMeeting.png?versionId=3939)
Following a virtual meeting in 2020, we are delighted to announce that the fifth iteration of our popular Journal Meeting will be held from 11-14 September 2022 at the historic Wotton House, Surrey. Registration is open now.
Preprints in Development
(update)-InPreprints.png?versionId=3939)
As part of our efforts to support the use of preprints and help curate the preprint literature, we are delighted to launch a new article type: ‘In preprints’. These pieces will discuss one or more recent preprints and place them in a broader context.
Submit your next Techniques and Resources paper to Development
-TechniquesAndResources.png?versionId=3939)
Development regularly publishes Techniques and Resources papers. These manuscripts describe a novel technique, a substantial advance of an existing technique, or a new resource that will have a significant impact on developmental biology research. Find out more here.
Transitions in development: Rashmi Priya
(update)-RashmiPriya.png?versionId=3939)
Rashmi Priya’s research group uses the zebrafish heart as a model system to understand the complex morphogenetic events of organogenesis. We interviewed Rashmi to learn about her career path so far, and to discuss the challenges of starting a lab in the middle of a global pandemic.
The Node Network
-NodeNetwork.png?versionId=3939)
The Node Network is a global directory of developmental and stem cell biologists, designed to help you find speakers, referees, panel members and potential collaborators. Find out more about the Node Network.