Advancing stem cell technologies for conservation of wildlife biodiversity

We are currently witnessing the sixth mass extinction event for life on Earth, posing unprecedented challenges for conservation biology. In contrast to previous extinction events, human-driven species losses are occurring exceptionally rapidly. Extinctions are estimated to be hundreds or thousands of times higher than expected background rates and have the potential to irrevocably alter the biosphere (Ceballos and Ehrlich, 2023). The scale and pace of this event demands concerted action from all areas of conservation biology to curb a catastrophic loss of biodiversity (Ceballos and Ehrlich, 2023). We propose that stem cell-associated techniques and their potential to develop new avenues for assisted reproductive technologies (ART) can complement traditional conservation approaches (such as habitat restoration and species monitoring; Fig. 1) and may play an important role in countering the effects of this extinction crisis (Hildebrandt et al., 2021; Saragusty et al., 2016). Pluripotent stem cells (PSCs) are a promising addition to the conservation toolkit, with the potential to become any cell type within an organism. PSCs can be derived directly from embryos or by converting somatic cells to induced pluripotent stem cells (iPSCs) (Evans and Kaufman, 1981; Martin, 1981; Takahashi and Yamanaka, 2006). Obtaining embryos for PSC generation is challenging for most mammalian species (Bolton et al., 2022), but iPSCs offer an alternative way to harness the same developmental potential for multiple target species and are suggested to be functionally equivalent to embryo-derived PSCs (Yamanaka, 2012).

Since their first derivation in 2006, iPSCs have been recognised for their potential to transform the fields of regenerative medicine, disease modelling and reproduction (Takahashi and Yamanaka, 2006). They also offer promise for protecting our planet's wildlife, as reprogramming technology offers a way to transform primary cells into an unlimited resource with a wide array of downstream applications such as in vitro gametogenesis and disease and embryo modelling. These applications could be leveraged to support traditional conservation techniques (Fig. 1) (Mooney et al., 2023). For example, biobanks are currently freezing both gametes and primary cell lines to safeguard the genetic diversity of potentially all (endangered) species for future biodiversity restoration efforts (Ballou et al., 2023; Bolton et al., 2022). However, cryopreserved cell lines are limited in utility, and reproductive material is challenging to obtain and preserve (Hildebrandt et al., 2021). In vitro gametogenesis could complement these biobanking efforts, as the ability to produce germ cells and embryos from biobank samples, independent of individual animals, would increase recovery options in extreme endangerment cases, such as for the northern white rhinoceros (Ceratotherium simum cottoni). Importantly, iPSCs also offer a route to modified offspring, delivering loss- and gain-of-function models, essential for functional genomics and performing facilitated adaptation (Thomas et al., 2013). Optimised, standardised and accessible reprogramming protocols applicable to a range of species will mark a new era in applied biobanking, providing the means to store valuable genomic information in a pluripotent form as stem cell ‘zoos’ (Lázaro et al., 2023).

The first iPSCs from endangered species were reported thirteen years ago (Ben-Nun et al., 2011), and subsequent studies have expanded this approach to other endangered animals (Ben-Nun et al., 2011,;Endo et al., 2022,;Hildebrandt et al., 2018,;Honda et al., 2017,;Katayama et al., 2022,;Ramaswamy et al., 2015,;Sukparangsi et al., 2022,;Verma et al., 2012, 2013,;Weeratunga et al., 2018,;Whitworth et al., 2019,;Zywitza et al., 2022,). However, there has been only slow progress toward integrating stem cell technologies within conservation. To address this challenge, the recent Stem Cell Technology for Genetic Rescue workshop, held in La Jolla, California in September 2023 and hosted by Revive & Restore, brought together scientists from diverse fields with the aim of deepening understanding surrounding species-specificities in pluripotency and differentiation. Participants explored the capacity for iPSC technologies to support traditional conservation efforts, highlighting the involvement of stem cell scientists as valuable contributors to the development of genetic rescue strategies for endangered species. In this Spotlight, we discuss the core focus areas selected during the workshop for their capacity to support applied biobanking and animal biodiversity restoration: reprogramming, in vitro gametogenesis, and disease and embryo modelling. Although we do not discuss plants here, stem cell technology also has the potential to support the conservation of plant species with seeds that cannot be preserved or for which cell culture is challenging (Greb and Lohmann, 2016). We call on the scientific community to prioritise the development of improved, reproducible and more accessible protocols for stem cell and associated technologies for the conservation of biodiversity.

Research into iPSC derivation has primarily focused on species with clinical, evolutionary or agricultural significance. For example, bat iPSCs were recently used to explore tolerance for high viral load with implications for COVID-19 (Déjosez et al., 2023), primate iPSCs are used as tools to unpack human evolution (Gallego Romero et al., 2015) and attempts to optimise the challenging process of bovine reprogramming have persisted largely due to the agricultural relevance of cattle (Déjosez et al., 2023; Gallego Romero et al., 2015; Pillai et al., 2021). Although there has been a gradual increase in the reported derivation of pluripotent cells from endangered species, these efforts cannot access funding reserved for biomedical applications and usually proceed no further than proof-of-concept demonstrations.

Although the core regulatory network for maintaining pluripotency, including the transcription factors OCT3/4, SOX2 and NANOG, is well-documented in vertebrates (Endo et al., 2020), differences in signalling pathways and isoforms across species underscore the need for further exploration (Fu et al., 2018; Kumar et al., 2022). In addition, when aiming to establish defined states of pluripotency, such as naive (pre-implantation) and primed (post-implantation), distinct signalling requirements become apparent (Marks et al., 2012; Nichols and Smith, 2009). In mouse PSCs, WNT signalling promotes naive pluripotency, whereas inhibition of WNT signalling supports human naive pluripotency (Bredenkamp et al., 2019; Ying et al., 2008). As a result, optimising reprogramming protocols can be labour-intensive, with each species requiring adjustments in methodology. For example, felid reprogramming is enhanced by the addition of NANOG (Verma et al., 2012), whereas successful platypus iPSC production has typically involved the presence of leukaemia inhibitory factor, basic fibroblast growth factor and a range of inhibitors targeting the MEK, ALK, GSKβ and TGFβ pathways (Whitworth et al., 2019). Variable results have been reported for hypoxic conditions in cattle and rabbits (Bessi et al., 2021; Honda et al., 2010) and the use of knock-out serum replacement (KOSR) instead of foetal bovine serum (FBS) promotes rhesus monkey reprogramming (Liu et al., 2008). Some species present additional challenges, appearing to be resistant to reprogramming (Kuzma-Hunt et al., 2023; Pillai et al., 2019). This may be because of epigenetic barriers, as reported for the naked mole rat (Tan et al., 2017), or multiple copies of the tumour suppressor gene p53, as is the case in the elephant (Appleton et al., 2024 preprint). Here, the SV40 large T-antigen was used to modulate p53 levels (Appleton et al., 2024 preprint). Overexpression of the SV40 large T-antigen has also been used to overcome reprogramming barriers observed for goat and sheep by dramatically increasing proliferation (Bao et al., 2011; Mali et al., 2008; Ren et al., 2011). Substantial variation in gene expression, as well as the capacity for chimerism and germline contribution, has been observed for derived iPSCs across species (Lee et al., 2017; West et al., 2011); however, because of methodological differences, it is unclear whether these discrepancies originate in inconsistent benchmarking, species-specific variation or protocol alterations. Standardised validation and improved understanding of pluripotency state transitions, as well as refining and manipulating culture conditions, will be key to producing high-quality iPSCs across taxa that can be used for conservation.

The delivery method for the reprogramming factors must also be considered. Non-integrative vectors, such as Sendai virus, mRNA or the reprogramming factor proteins themselves, maintain genomic integrity and are suitable for applications in biodiversity conservation (Nishimura et al., 2011; Okita et al., 2011). When reprogramming fails, species-specific transcription factors offer an alternative to using human or mouse factors (Appleton et al., 2024 preprint; Liu et al., 2008). Owing to their unlimited self-renewal capacity, iPSC cultures are prone to accumulating genetic mutations, so the maintenance of genomic integrity is both challenging and essential (Endo et al., 2022; Koh et al., 2013; Park et al., 2015). Regular monitoring can be performed using available methods, including karyotyping, genome sequencing and chromosome mapping, which currently represent the most effective approaches for detecting these aberrations (Ludwig et al., 2023). However, even iPSCs harbouring proliferation-associated mutations can still be valuable as research tools, offering insights into differentiation pathways and disease mechanisms, and serving as a repository for the genetic diversity of species (Lee et al., 2017; Noto et al., 2014; Song et al., 2021).

Although many challenges remain, we note that reprogramming efforts for conservation would greatly benefit from expanded access to cell lines and insights gained from emerging fields such as cellular agriculture, alongside enhanced resources for endangered species and optimised derivation processes (Box 1).

After generating PSC lines, it is crucial to validate them as such using standardised benchmarking. This can be achieved by assessing different criteria such as morphology, self-renewal, gene expression levels, protein levels, methylation states and silencing of ectopic reprogramming factors (Boroviak and Nichols, 2017; Ying and Smith, 2017). However, the ultimate test of pluripotency is to demonstrate germline transmission by creating chimaeras (incorporating PSCs into an embryo of another individual) that contain PSC-derived germ cells, which is not possible without established reproductive technologies and access to embryos (Bradley et al., 1984; Okita, 2007). Although key features of pluripotency have been extensively characterised in humans and rodents (Du and Wu, 2024; Smith, 2001), the validation of pluripotency in non-model species remains challenging. This is largely due to a lack of species-specific antibodies, availability of reference genomes (Wang et al., 2021 preprint) and challenges in obtaining embryos for comparison.

Conventional assays for validating pluripotency may not be available for endangered species, making it essential to establish realistic benchmarking standards. These standards should include evidence of differentiation into all three germ layers, as outlined by the ISSCR guidelines (https://www.isscr.org/standards-document). One approach is the transplantation of putative PSCs into immunodeficient mice to assess teratoma formation, which would confirm differentiation into endodermal, mesodermal and ectodermal derivatives (Evans and Kaufman, 1981; Martin, 1981). Alternatively, in vitro tri-lineage differentiation can be demonstrated using embryoid body assays, where PSCs are cultured in suspension to form spherical aggregates that differentiate into various cell types (Desbaillets et al., 2000; Doetschman et al., 1985). The use of transcriptomic atlases may also provide a valuable tool for characterising pluripotency and validating differentiation outcomes (Malkowska et al., 2022). Finally, interspecies chimera technology offers an alternative route for in vivo differentiation and germline transmission tracking (Wu et al., 2017). This method involves integrating PSCs from endangered species into embryos of more readily available model organisms, potentially overcoming the limitations of traditional validation approaches.

In vitro gametogenesis (IVG) involves generating spermatozoa or oocytes outside of a living organism (Saitou and Hayashi, 2021), paving the way for assisted reproductive technologies such as in vitro fertilisation (IVF) or intracytoplasmic sperm injection (ICSI) to induce fertilisation and produce embryos. The latest innovations in murine IVG research might offer solutions for the most hopeless situations where genetic material may only be available for one sex, as XY chromosomes can now be converted into XX in pluripotent stem cells and deployed for in vitro oogenesis (Murakami et al., 2023). Complete IVG has been achieved only in mice but it does provide proof-of-concept for using this approach in other species (Hayashi et al., 2012; Saitou and Hayashi, 2021; Yoshino et al., 2021). Unlike embryo modelling or cloning, this technology enables sexual reproduction and recombination, producing new genetic profiles (Cowl et al., 2024). This is an important advantage because dwindling populations experience reduced genetic diversity.

The derivation of gametes from iPSCs is an integral part of the strategy to save the northern white rhinoceros (Hayashi et al., 2022; Hildebrandt et al., 2021; Korody et al., 2021; Saragusty et al., 2016). This project is ongoing, but has already established primordial germ cell-like cells (PGCLCs) from northern white rhinoceros iPSCs (Hayashi et al., 2022; Korody et al., 2021). However, a solid understanding of the reproductive physiology of any target species will be fundamental to achieving healthy live births (Comizzoli and Holt, 2019; Herrick, 2019; Mastromonaco and Songsasen, 2020). Although the closely related southern white rhinoceros can provide such information for the northern white rhinoceros, not every species has a readily available close relative. Bridging the gap between the promising results obtained in mice and IVG for endangered species will require optimisation in large domestic animal models such as pigs or cattle, as well as non-human primates (Gyobu-Motani et al., 2023; Seita et al., 2023). This approach also requires a robust understanding of the molecular pathways that regulate the generation of germ and supporting cells (such as granulosa and Sertoli cells) in vitro. Initial IVG efforts relied on access to primary supporting cells, but recent work demonstrates the feasibility of deriving these directly from iPSCs (Yoshino et al., 2021).

Notably, IVG technology could also be used to generate spermatogonial stem and progenitor cells (SSPCs) for use in conservation biology. In mice and livestock (pigs, goats and sheep) SSPCs can be transplanted into the testes of recipient males (Ciccarelli et al., 2020; Zhao et al., 2021a) and these can then be returned to the population to spread new or lost diversity. This approach could also be used to introduce genetic modifications into endangered animals without requiring full IVG protocols or ART for every species.

As iPSC technology continues to advance, IVG provides a promising way to ensure the reproductive viability of threatened species. While these advanced artificial reproductive techniques are developed, biobanking provides a buffer across time to store not only genomic information, but also precious cellular material (Hildebrandt et al., 2021). Although cryopreservation of germ cells such as spermatozoa and oocytes would facilitate immediate fertilisation, biobanks often focus on collection of tissue samples and cell lines because of practical and technical limitations of cryopreservation methods (Bolton et al., 2022; Hildebrandt et al., 2021). IVG will play a key role in using these somatic tissues for reproductive purposes. However, the use of stem cell-associated reproductive technologies poses challenges, so these approaches should be initiated early and in parallel with enhanced cryopreservation techniques for continued banking of reproductive material. For example, germ cells derived from iPSCs have not been regulated by germline protective mechanisms and are more vulnerable to mutation (Saitou and Hayashi, 2021), so their (epi)genetic quality must be examined closely. Indeed, offspring born from these sources may exhibit genetic abnormalities and replicating the epigenetic state of primordial germ cells in vitro remains challenging (Bhartiya et al., 2014). Leveraging precursors of the endogenous pre-existing stem cells within the ovary (oogonial stem cells) could help address this caveat, as these cells express specific markers and exhibit the epigenetic profile of primordial germ cells (Bhartiya et al., 2014). In mice, these ovarian stem cells survive oncotherapy, differentiate into oocyte-like structures and result in healthy offspring (Zou et al., 2009). However, iPSC technology remains crucial in scenarios where ovarian tissue is absent.

In addition to their potential for generating gametes in vitro, iPSCs can serve as an unlimited source of differentiated somatic cell types for deployment in developing effective monitoring and mitigating strategies for disease, toxins and other environmental challenges.

Understanding barriers to disease transmission is essential for protecting vulnerable populations. iPSCs from susceptible wild species could provide relevant cell types to understand the basis of disease resilience and susceptibility and develop potential therapeutic or prophylactic measures. This might prove particularly useful in safeguarding wild bird populations from avian flu, wild dogs and carnivores from distemper, or wild pigs from African Swine Fever Virus. iPSCs could also generate elements of the immune system and pathogen-targeted tissues to develop culture and three-dimensional (3D) organoid experimental systems that more accurately model disease phenotypes (Sharma et al., 2020). For example, horse iPSC-derived neurons have been used to investigate susceptibility to neurotropic Flavivirus infection (Fortuna et al., 2018), and pig PSC-derived macrophages present new opportunities to investigate resilience to pathogens such as African Swine Fever Virus that threaten both domestic pig and endangered wild pig populations (Meek et al., 2022). Fine-tuning PSC differentiation protocols to generate phenotypically relevant cells at scale will be a major future challenge in maximising the utility of these disease studies. This will involve translating and optimising existing protocols, as well as the generation of new methods.

In the context of long-term changes in global temperatures and weather patterns, understanding resilience against environmental change is also crucial. A pioneering study, comparing human and hibernating ground squirrel iPSC-derived neurons, identified key biochemical stress pathways that, when modulated appropriately, improved resistance to thermal stress in iPSC-derived neurons from both humans and rats (Ou et al., 2018). Understanding mechanisms underpinning resilience is particularly important for developing strategies aimed at protecting the keystone species in threatened communities such as corals, which we discuss in more detail below.

The exceptional ability of PSCs to organise themselves into complex structures has driven significant advancements in creating 3D structures known as stem cell-based embryo models (SCBEMs) that replicate various early mammalian developmental stages, from pre-implantation through to the beginning of organ formation (Wu and Fu, 2024). These exhibit varying degrees of resemblance to actual embryos in terms of shape, overall gene expression profiles and cellular composition (Wu and Fu, 2024). One of the most promising applications of SCBEMs for species preservation is the creation of pre-implantation blastocyst models, called ‘blastoids’ (Oura et al., 2023), for reproductive purposes, which will be the focus of discussion here. Notably, SCBEMs offer the potential for genetic rescue and broader biodiversity conservation efforts through the generation of reproductive cells; however, these applications will not be discussed here.

In recent years, blastoid models have been created across a variety of mammalian species, including mice (Li et al., 2019; Rivron et al., 2018; Sozen et al., 2019), humans (Kagawa et al., 2021; Yanagida et al., 2021; Yu et al., 2021), cattle (Pinzón-Arteaga et al., 2023), pigs (Xiang et al., 2024), monkeys (Li et al., 2023) and bats (Déjosez et al., 2023). These models effectively replicate the essential cell types needed for both the development of the foetus and the tissues that support it, such as the trophectoderm and hypoblast. Blastoids are produced through various methods: they can be formed by guiding a single type of embryo-derived PSC to generate both the embryo and the support tissues (Li et al., 2019; Yu et al., 2021), by mixing embryo-derived PSCs with cells destined to become support tissues (Pinzón-Arteaga et al., 2023; Rivron et al., 2018) or through reprogramming of somatic cells to make iPSCs that can be used as a starting population (Liu et al., 2021). Murine (Li et al., 2019; Rivron et al., 2018), monkey (Li et al., 2023) and bovine (Pinzón-Arteaga et al., 2023) blastoids placed into surrogate mothers can initiate early stages of pregnancy. However, blastoids transferred to the uterus have not yet developed sufficiently to result in the birth of offspring. As implantation might be the bottleneck, in vitro platforms could facilitate improvements at the endometrial-blastoid interface (Shibata et al., 2024). To date, no blastoids have been developed for endangered species, representing an unexplored area of potential. To move closer to this objective, the field must advance our knowledge of early development in different species, establishing more effective embryo cultures (Aguilera-Castrejon et al., 2021) and PSC conditions (Du and Wu, 2024), as well as refining reprogramming methods (MacCarthy et al., 2024). Such advancements are crucial for harnessing the full potential of SCBEM technology.

The extensive groundwork already done in mice and humans, along with the availability of ARTs, suggests that implementing stem cell approaches is most achievable for mammalian endangered species. However, recent advancements in PSC research are expanding the possibilities across a broader range of taxa. The following sections discuss the potential of stem cell technologies beyond mammals, including avian species, non-avian reptiles and amphibians, and marine invertebrate species.

About 12% of avian species are currently threatened with extinction (www.iucnredlist.org). Both embryo-derived PSCs and iPSCs have been obtained for avian species, exhibiting similarities in gene regulatory networks to mammals (Intarapat and Stern, 2013). Avian species present an advantageous system for embryonic integration as stem cells can be injected directly into the embryo within the egg to generate chimaeras (Intarapat and Stern, 2013). Recently, iPSCs from four endangered avian species were generated using standard reprogramming factors, plus KLF2 and YAP (Katayama et al., 2022). Although the derived cells expressed core pluripotency factors including POU5F1, LIN28A/B and NANOG, gene expression and pathway analysis differed from the standard murine profile. SOX3 was more highly expressed than SOX2, highlighting its active role in avian pluripotency (Whitworth et al., 2019). iPSCs derived from the Japanese ptarmigan could integrate into chicken embryos and produce interspecific chimeras, although germline competence was not observed (Katayama et al., 2022). Developing protocols for germline-competent avian PSCs would be a significant breakthrough in avian transgenesis, holding immense promise for conservation. Differentiation of avian iPSCs to primordial germ cells in vitro, followed by embryo-injection via the egg to complete gametogenesis in vivo, will address avian-specific challenges for performing IVF by facilitating natural reproduction. However, the germline-restricted chromosome in songbirds poses a challenge for using somatic cells as a starting point (Borodin, 2023).

Reptile and amphibian populations are experiencing sharp declines worldwide (Strand et al., 2020). Although iPSC technology has not yet been reported for non-avian reptiles, stem cell-derived organoids from snakes represent advances for this taxon (Post et al., 2020). As numerous reptiles exhibit temperature-dependent developmental and physiological processes, the derivation of stem cells could offer an avenue to explore the impact of climate change on these species. Similarly, although amphibian reprogramming remains unexplored, it holds the potential to facilitate disease modelling, particularly in response to the deadly chytrid fungus (Bolton et al., 2022). iPSC cultures might improve amphibian cell yield and utility for downstream research (Strand et al., 2020). Interestingly, intramuscular injection of the Yamanaka factors in tadpoles results in upregulation of core pluripotency markers, suggesting conservation of Yamanaka-induced reprogramming for this taxon (Vivien et al., 2012).

Marine invertebrates represent a substantial portion of global biodiversity (Bodnar, 2016; Chen, 2021). Currently, reef-building corals are under severe threat from increasing ocean temperatures that lead to bleaching. Because coral responses to stress vary (Palumbi et al., 2014; van Oppen et al., 2018), one conservation goal has been imparting stress-resilient genotypes (National Academies of Sciences, Engineering, and Medicine, 2019). Transferring genetic properties from one coral to another or, after manipulation, back to the same coral, requires the ability to isolate stem-like progenitor cells and engraft them through transplantation. Preliminary work suggests that candidate stem cells in the sea anemone Nematostella vectensis can proliferate and integrate, contributing to gene and phenotype transfer, cell differentiation and longevity, for genetic rescue (Talice et al., 2023). Many marine invertebrates exhibit indeterminate growth, high regenerative capacity and asexual modes of reproduction, suggesting robust stem cell-like properties. However, little is known about their stem cell biology (Ballarin, Rinkevich and Hobmayer, 2022). The ability to culture stem cells from marine invertebrates would provide a powerful resource for understanding their biology, symbiosis, disease aetiology and stress response, and could even provide an alternative to wild harvest through cellular agriculture (Rubio et al., 2019). A collaborative effort is needed to develop an integrated systems-level approach to optimise in vitro culture conditions, devise markers to validate cell identity and prioritise taxa for which cell culture tools can address the most pressing problems facing marine ecosystems.

Translating iPSC technology to wildlife conservation might provide a way to both safeguard and produce resilient individuals from endangered animal species. It is, therefore, paramount to fund and develop parallel methods for germ cell derivation, as well as apply clinical stem cell research to wildlife disease. Learning from non-mammalian species and harnessing their developmental potential will deliver essential insight into the effects of the climate crisis, as well as provide solutions to protect the Earth's biodiversity.

The Stem Cell Technology for Genetic Rescue 2023 workshop aimed to accelerate advancements and foster collaborations in this rapidly evolving field. Workshop participants identified key barriers, including a lack of funding and the fragmentation of research within the conservation community. In contrast to the frequent convening of experts in biomedicine, stem cell researchers working on endangered species often operate in isolation due to the lack of dedicated scientific meetings. Enhancing research transparency and fostering cross-disciplinary knowledge sharing is essential to expedite progress and prevent redundancy.

Currently, the literature prioritises close alignment of results with the mouse model, limiting the exploration of pluripotency as a biological property and an evolutionary feature. Standardising both reprogramming methods and approaches to benchmarking will enable accurate comparisons across taxa. For example, there is a clear need to improve and align transcriptomic resources for PSC gene regulatory networks across species to deepen our understanding of pluripotency. This may include standardising RNA sequencing methods (Ramsköld et al., 2012) and will depend on generating robust reference genomes for more species. Comparing expression profiles across proven pluripotent stem cells from a wide diversity of species could be used to define broadly conserved networks associated with pluripotency (Déjosez et al., 2023; Kumar et al., 2022; Whitworth et al., 2019). Open access to transcriptomic profiles for reprogrammed cells across species will be crucial for expanding knowledge of pluripotency regulation across evolutionary time, and integrating this information as a landscape for stem cell states in vertebrates is a first vital step toward developing a more universal reprogramming toolkit. In future, this technology could also be applied to more diverged groups, such as invertebrates.

Stem cell technology has the capacity to augment traditional conservation efforts, as biobanking and advanced ART offer emergency measures to conserve both species and genetic material. However, their impact and measurable effects on conservation remain to be determined (Sutherland et al., 2021). Therefore, these technologies and approaches should be integrated with and funded alongside established conservation approaches. As habitat restoration and climate action struggle to keep pace with rapid species decline, stem cell-associated techniques offer an additional buffer to mitigate extinctions. Leveraging this potential will complement current conservation efforts to safeguard species diversity. Ultimately, however, as with any conservation measure, continued protection of suitable habitats for wildlife will be essential for maintaining a healthy, resilient and biodiverse planet.

We thank the Revive & Restore staff and Ryan Phelan for funding, planning and hosting the Stem Cell Technology for Genetic Rescue workshop. The workshop was supported by: Gold Sponsors – The Pershing Square Foundation, Promega Corporation and San Diego Zoo Wildlife Alliance; and Bronze Sponsors – Occam Biosciences, Viagen Pets & Equine, Conception Bio and Vet-Stem. We thank the workshop organisers: Ashlee Hutchinson, Bridget Baumgartner, Marmee Manack, Pierre Comizzoli, Jeanne Loring, Monique Paris, Andrew Pask, Gareth Sullivan, Deanne Whitworth, Suzannah Williams and Jun Wu. We additionally thank the Workshop Panellists: Pae Wu, Owain Edwards, Bridget Baumgartner, Ben Novak, Pete Miraglia and Linda Penfold. Special thanks to Marmee Manack, Traci Eckels and Jaqueline Mountcastle for assistance with logistics. We also thank Samantha Zwicker and Dylan Singer for critical reading of the manuscript.