The expression of the floral regulators LEAFY, APETALA1 and AGAMOUS-LIKE8 was examined during light treatments that induced flowering in Arabidopsis, and was compared to time points at which floral determination occurred. Extension of an 8-hour day by either continuous red- or far-red-enriched light induced LEAFY and AGAMOUS-LIKE8 expression within 4 hours. The 4 hours of additional light was sufficient for floral determination only in the far-red-enriched conditions, while 12–16 hours of additional light was required for floral determination in the red-enriched conditions. These results indicate that the induction of floral regulatory genes and induction of flower formation can be uncoupled under certain circumstances. Expression of LEAFY and AGAMOUS-LIKE8 in the shoot apex at the time of floral determination is also consistent with genetic data indicating that these genes are involved in the first steps of the transition from vegetative to reproductive development. In contrast to LEAFY and AGAMOUS-LIKE8, APETALA1 expression was first observed 16 hours after the start of photoinduction. Since this time point was always after floral determination, APETALA1 is an indicator of floral determination.
JOURNAL ARTICLE|
01 October 1997
Floral determination and expression of floral regulatory genes in Arabidopsis
F.D. Hempel,
F.D. Hempel
Department of Plant and Microbial Biology, University of California, Berkeley 94720-3102, USA. evoke@nature.berkeley.edu
Search for other works by this author on:
D. Weigel,
D. Weigel
Department of Plant and Microbial Biology, University of California, Berkeley 94720-3102, USA. evoke@nature.berkeley.edu
Search for other works by this author on:
M.A. Mandel,
M.A. Mandel
Department of Plant and Microbial Biology, University of California, Berkeley 94720-3102, USA. evoke@nature.berkeley.edu
Search for other works by this author on:
G. Ditta,
G. Ditta
Department of Plant and Microbial Biology, University of California, Berkeley 94720-3102, USA. evoke@nature.berkeley.edu
Search for other works by this author on:
P.C. Zambryski,
P.C. Zambryski
Department of Plant and Microbial Biology, University of California, Berkeley 94720-3102, USA. evoke@nature.berkeley.edu
Search for other works by this author on:
L.J. Feldman,
L.J. Feldman
Department of Plant and Microbial Biology, University of California, Berkeley 94720-3102, USA. evoke@nature.berkeley.edu
Search for other works by this author on:
M.F. Yanofsky
M.F. Yanofsky
Department of Plant and Microbial Biology, University of California, Berkeley 94720-3102, USA. evoke@nature.berkeley.edu
Search for other works by this author on:
F.D. Hempel
Department of Plant and Microbial Biology, University of California, Berkeley 94720-3102, USA. evoke@nature.berkeley.edu
D. Weigel
Department of Plant and Microbial Biology, University of California, Berkeley 94720-3102, USA. evoke@nature.berkeley.edu
M.A. Mandel
Department of Plant and Microbial Biology, University of California, Berkeley 94720-3102, USA. evoke@nature.berkeley.edu
G. Ditta
Department of Plant and Microbial Biology, University of California, Berkeley 94720-3102, USA. evoke@nature.berkeley.edu
P.C. Zambryski
Department of Plant and Microbial Biology, University of California, Berkeley 94720-3102, USA. evoke@nature.berkeley.edu
L.J. Feldman
Department of Plant and Microbial Biology, University of California, Berkeley 94720-3102, USA. evoke@nature.berkeley.edu
M.F. Yanofsky
Department of Plant and Microbial Biology, University of California, Berkeley 94720-3102, USA. evoke@nature.berkeley.edu
Online Issn: 1477-9129
Print Issn: 0950-1991
© 1997 by Company of Biologists
1997
Development (1997) 124 (19): 3845–3853.
Citation
F.D. Hempel, D. Weigel, M.A. Mandel, G. Ditta, P.C. Zambryski, L.J. Feldman, M.F. Yanofsky; Floral determination and expression of floral regulatory genes in Arabidopsis. Development 1 October 1997; 124 (19): 3845–3853. doi: https://doi.org/10.1242/dev.124.19.3845
Download citation file:
-HUBSwebinar.jpg?versionId=4486)
(update)-InPreprints.png?versionId=4486)
