The introduction of recombinant DNA into mouse embryos has proved to be a useful technique in addressing a number of important developmental questions, e.g. the identification of DNA sequences controlling tissue-specific gene expression and the role of genes in growth control (for reviews see Palmiter & Brinster, 1985; Wagner & Stewart, 1986). Currently the favoured method for gene transfer into mice is by DNA injection into a pronucleus of the fertilized egg. However, an alternative method is to exploit retroviruses as vectors for introducing genes either directly into embryos or into embryonal carcinoma (EC) or embryonic stem (ES) cells which can then be used to form chimaeric mice (Bradley, Evans, Kaufman & Robertson, 1984). There are a number of advantages to using retroviral vectors, the major one being that they can infect a wide variety of cells at an efficiency approaching 100 %. In several instances...
The expression of retroviral vectors in murine stem cells and transgenic mice
- Views Icon Views
-
Article Versions Icon
Versions
- Version of Record 01 October 1986
- Share Icon Share
-
Tools Icon
Tools
- Search Site
Colin L. Stewart, Ulrich Rüther, Christa Garber, Mirka Vanek, Erwin F. Wagner; The expression of retroviral vectors in murine stem cells and transgenic mice. Development 1 October 1986; 97 (Supplement): 263–275. doi: https://doi.org/10.1242/dev.97.Supplement.263
Download citation file:
Sign in
Client Account
Sign in via your institution
Sign in via ShibbolethAdvertisement
Cited by
About us

Our publisher, The Company of Biologists, turns 100 this year. Read about the history of the Company and find out what Sarah Bray, our Chair of the Board of Directors, has to say.
Biologists @ 100 - join us in Liverpool in March 2025

We are excited to invite you to a unique scientific conference, celebrating the 100-year anniversary of The Company of Biologists, and bringing together our different communities. The conference will incorporate the Spring Meetings of the BSCB and the BSDB, the JEB Symposium Sensory Perception in a Changing World and a DMM programme on antimicrobial resistance. Find out more and register by 28 February 2025 to join us in March 2025 in Liverpool, UK.
Call for papers – Lifelong Development: the Maintenance, Regeneration and Plasticity of Tissues

Development invites you to submit your latest research to our upcoming special issue – Lifelong Development: the Maintenance, Regeneration and Plasticity of Tissues. This issue will be coordinated by Guest Editors Meritxell Huch (Max Planck Institute of Molecular Cell Biology and Genetics, Germany) and Mansi Srivastava (Harvard University and Museum of Comparative Zoology, USA), working alongside our team of academic Editors. Submit your articles by 15 May 2025.
A case for broadening our view of mechanism in developmental biology

In this Perspective, B. Duygu Özpolat and colleagues survey researchers on their views on what it takes to infer mechanism in developmental biology. They examine what factors shape our idea of what we mean by ‘mechanism’ and suggest a path forward that embraces a broad outlook on the diversity of studies that advance knowledge in our field.
In preprints
Did you know that Development publishes perspectives on recent preprints? These articles help our readers navigate the ever-growing preprint literature. We welcome proposals for ‘In preprints’ articles, so please do get in touch if you’d like to contribute.