Y. Andeol1, J. David2and J. Signoret1. 1Laboratoire de biologie du Développement, Université de Caen, 14032 Caen, France. 2Laboratoire de Biochimie du Développement, Université de Rennes 1, Campus Beaulieu, 35042 Rennes, France

A heavy form of DNA ligase (8S) replaces the light form (6S) of enzyme when Axolotl egg is fertilized or artificially activated at 18 °C. When eggs either fertilized or activated are maintained at 10 °C the heavy form of enzyme does not appear and the light DNA ligase remains undiminished.

Fertilized eggs develop normally, although slowly, at 10 °C with but the light molecular form of DNA ligase, and no trace of the heavy form reported as specific of developmental stages at 18 °C. Thus, considering that the two molecular forms of DNA ligase are involved normally in different processes as it is generally assumed, we must admit that...

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