ABSTRACT
Three haemoglobins were isolated by ion-exchange chromatography from the haemolysates of embryonic duck erythrocytes up to 8 days of development. The component globins were characterized both by electrophoresis in dissociating conditions and by finger-printing analysis. The major haemoglobin fraction El appears to be an embryonic tetramer since its constituent globins are different from all the others synthesized during embryonic and adult life. The two minor fractions E2 and E3 show a-type subunits that are very similar to those of the two adult haemoglobins Al and A2 respectively. They are present all through embryonic life, as demonstrated by chromatographic analysis. For these reasons they have been considered foetal.
The two haemoglobins typical of the adult animal are found in the red cells of the embryo from 8 days of incubation. Their relative amounts change continuously during embryonic development and reach the adult value after hatching.
