ABSTRACT
For several years in this laboratory, ultra-violet light has been used to produce haploid amphibian embryos, sometimes to provide haploid nuclei for transplantation experiments, as in Pantelouris & Jacob (1958). The ultra-violet method gives a high proportion of haploids, is very simple and speedy to carry out and involves the use of only inexpensive apparatus. Our method and results are described here to help others choose between this and alternative methods such as the use of toluidine blue by Briggs (1952), or X-rays by Lehman (1955) and Rugh (1939). Other methods are reviewed in Fankhauser (1937) and Drebinger (1951).
Copyright © 1958 by Company of Biologists
1958
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