ABSTRACT
Rhesus monkey follicular oocytes obtained from gonadotropin-injected animals were treated with hyaluronidase and cultured in Whitten’s medium. Of 74 oocytes beyond germinal vesicle stage, 57 were activated. Twenty-one of the activated oocytes cleaved one to three times. Behavior in culture and the values of relative Feulgen-DNA content in nuclei of six normally cleaved 6-to 10-cell parthenogenones showed that four resulted from the activation of primary oocytes, one from a secondary oocyte, and one from a primary polyploid oocyte. One parthenogenone with four pronuclei, and two with a single pronucleus (without extrusion of the first and second polar bodies in either case) were also observed.
Copyright © 1977 by Company of Biologists
1977
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