ABSTRACT
Females of Drosophila melanogaster were centrifuged at 2400 g and 4200 g for 3 h in a number of different orientations. The oocytes in various stages of vitellogenesis become separated into three layers; a centrifugal yolk layer, a central cytoplasmic layer, and a centripetal lipid layer. The direction of layering is related to the orientation of the female. The process of recovery of the ovaries was followed and the development of the eggs laid analysed. Many of the eggs laid over the subsequent 3 days die very early in development and fail to produce any differentiated structures. Some hatch into normal larvae and others produce defective embryos. The most common defect being a misarrangement of the segmentation. The differences in the kinds of experimentally inducible pattern aberrations in Drosophila and other diptera is discussed.
