ABSTRACT
The induction of a frequent fusion in dissociated fish embryonic cells is reported. Fusion was induced during mechanical isolation of blastomeres in normal saline solution or Ca2+-free saline solution by quickly bringing about physical contact between cells within about 1·5 min of dissociation. Fused blastomeres were obtained in about 18% yield from early morula cells, in about 68% yield from early blastula cells and in about 5% yield from early gastrula cells.
Copyright © 1977 by Company of Biologists
1977
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