In the accompanying paper (Slack, 1977), it is suggested that the normal anteroposterior pattern of the amphibian limb arises as a consequence of an interaction between the prospective limb and the flank. It has been shown that that part of the limb rudiment which is adjacent to flank tissue in the embryo becomes a posterior edge. So far we have considered neither the mechanism of this ‘posterior determination’, nor the mechanism of limb individuation, that is the process whereby cells in different anteroposterior positions differentiate to form a characteristic pattern of muscles and cartilages.

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