Immunofluorescent study of the distribution of adult neuro-specific antigens in the chick embryo

The adult neuro-specific antigens have been localized by immunofluorescence techniques in diencephalon and mesencephalon of chick embryo. This study has been made using fresh or fixed tissues from embryos 72,48 or 36 h old. At 72 h of incubation the wall of diencephalon shows marked fluorescence; at 48 h of incubation the fluorescent cells are localized in an outer layer and an inner one. In the48h-old embryo the reaction is more distinct and intensive in fresh tissues than in fixed tissues. At 36 h of incubation no fluorescence has been detected either in fresh tissues or in fixed tissues.

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