Tips from fruiting bodies and conuses were transplanted into interphase fields of Dictyostelium discoideum amoebae. Progressively increasing concentrations of beef-heart phosphodiesterase added to the fields significantly decreased the chemotactic range of the responding amoebae. The findings suggest that the tip secretes c-AMP. We also find that the chemotactic range is independent of the size of the tip implying that the tip may produce a regulating gradient.

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