ABSTRACT
Ultra-microfluorometric techniques were adapted to follow the time sequence of glycogen degradation during the differentiation of two cell types in Dictyostelium discoideum. Glycogen content, glycogen phosphorylase activity, and inorganic phosphate accumulation were localized in specific cell types during stalk and spore development.
Glycogen levels in pre-stalk cells remained constant during the pseudoplasmodium and early culmination stages of development. However, as pre-stalk cells migrated into the position of stalk formation, a cell specific degradation of glycogen was observed. The loss of glycogen from pre-stalk cells was accompanied by an increase in the activity of glycogen phosphorylase. This increase in activity from 0·04 to 0·14 moles/h/kg dry wt. occurred as pre-stalk cells entered the position of stalk formation. An inverse relationship was found between glycogen levels and inorganic phosphate (Pi) levels in the developing stalk. During the process of stalk construction, a gradient of Pi levels occurred from the apex to the base of the developing stalk. Glycogen degradation from pre-spore cells lagged behind that of pre-stalk cells. No change in pre-spore cell glycogen levels was observed until stalk construction was nearly completed. The results emphasize the importance of the physical position of a cell with respect to its composition and fate during development.
