ABSTRACT
Cleaving embryos of the sea urchin Paracentrotus lividus exposed briefly to buffered saline release materials into the medium which will bleach a yellow mixture of DTNB and DTT [5,5’ -dithiobis (2-nitrobenzoic acid) and dithiothreitol]. The decolorant activity is thermolabile and dialysable. The bleached mixture recolors rapidly on heating, suggesting the breakdown of a complex between the decolorant and the yellow DTNB ion.
The release of decolorants is maximal at the blastula stage, and in Ca-free saline, Tris-HCl pH 7. When various untreated embryonic stages are homogenized with DTNB, the assay for SH is lowest at blastula, suggesting that decolorants in the blastulae interfere with the assay.
Copyright © 1972 by Company of Biologists
1972
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