The uptake and transfer of radioactive compounds in axial grafts of Hydra Available to Purchase

Hydra littoralis and H. attenuata were labelled with ⁴⁵Ca, ³²PO₄, or [³H]thymidine, either by exposing them to a medium containing the isotope or by injecting the isotope into their gut cavities. Various portions of labelled and unlabelled hydra were grafted together in diverse combinations, and the rate of transfer of radioactivity to the unlabelled portion was assessed. The relative transfer rates of the isotopes were ³²PO₄ > ⁴⁵Ca²⁺ ⪢ [³H]thymidine. The transfer rates for ³²P-labelled compounds and ⁴⁵Ca were unaffected either by the polarity gradient (from head to foot and vice versa) or by the existence of potentially separate biological fields. Isotopes were not readily transferred between hydra or pieces of hydra which were in apposition but not grafted together. A rapid and sensitive method for the measurement of hydra mass is also described.