We have previously shown that human myoblasts do not fuse when their voltage fails to reach the domain of a window T-type Ca2+ current. We demonstrate, by changing the voltage in the window domain, that the Ca2+ signal initiating fusion is not of the all-or-none type, but can be graded and is interpreted as such by the differentiation program. This was carried out by exploiting the properties of human ether-à-go-go related gene K+ channels that we found to be expressed in human myoblasts. Methanesulfonanilide class III antiarrhythmic agents or antisense-RNA vectors were used to suppress completely ether-à-go-go related gene current. Both procedures induced a reproducible depolarization from -74 to -64 mV, precisely in the window domain where the T-type Ca2+ current increases with voltage. This 10 mV depolarization raised the cytoplasmic free Ca2+ concentration, and triggered a tenfold acceleration of myoblast fusion. Our results suggest that any mechanism able to modulate intracellular Ca2+ concentration could affect the rate of myoblast fusion.
Development presents…
Development is delighted to host a webinar series showcasing the latest developmental biology and stem cell research. The webinars are held each month with talks from postdocs applying for independent positions as part of our Pathway to Independence programme. Visit Development presents... on the Node to see which stimulating topics are coming up in the next few months.
Meet our 2025 Pathway to Independence (PI) fellows
We are delighted to announce our third cohort of PI fellows - researchers whom we will be supporting as they transition from postdoc to Principal Investigator. Read about the eight talented fellows chosen, whom we're excited to be working with as they navigate the job market.
A case for broadening our view of mechanism in developmental biology
In this Perspective, B. Duygu Özpolat and colleagues survey researchers on their views on what it takes to infer mechanism in developmental biology. They examine what factors shape our idea of what we mean by ‘mechanism’ and suggest a path forward that embraces a broad outlook on the diversity of studies that advance knowledge in our field.
Browse by subject
![Development logo - Browse by subject: Explore Development's content, now easily accessible by subject area. The ad has a black background with three vibrant scientific images: a developing embryo on the left, a green plant-like structure in the center, and a gastruloid (a circular cell with a bright pink center and blue outer ring) on the right. [Blue button: browse content].](https://cob.silverchair-cdn.com/ImageLibrary/Development/Snippets/2025_05_Dev_Browse-by-subject_600x230_Snippet.png?versionId=8993)
From cardiovascular development and regeneration to tissue engineering and organoids, Development’s browse by subject archive allows you to access the latest papers (from late 2024 onwards) on a particular field of interest. In addition to our curated subject collections, these subject pages allow readers to browse a broader range of papers organised by topic.
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