The method for determination of mass in biological material described by Engström & Lindström (1950) is founded on the fact that the absorption of X-radiation at 8-10 Å is proportional to the mass of the biological substance. In elaborating this method for quantitative analysis of nerve-cells and their components (Brattgård & Hydén, 1952) we found it important to consider the following sources of error:

  1. The reference system.

  2. The photographic process and the photometry.

  3. The treatment of the material.

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