Fig. 1. Effects of 4-day-long simulated heatwaves on survival and developmental duration of L4 larvae (A,C) and pupae (C,D). The controls (Ctrl, reared at constant 34°C, in blue) were compared to individuals that had been exposed to increased rearing temperatures at 37°C (in warm yellow) and 38°... More about this image found in Effects of 4-day-long simulated heatwaves on survival and developmental dur...

Fig. 2. Effects of 4-day-long simulated heatwaves during L4 stage on bumblebee morphometrics. (A) Relative body mass gain during treatment (means±s.e.). (B) Relative body mass loss during pupation (means±s.e.). (C-F) Consequences on their dry mass, ITD (intertegular distance), head width, and re... More about this image found in Effects of 4-day-long simulated heatwaves during L4 stage on bumblebee morp...

Fig. 3. In vitro rearing of L4 larvae. (A) Design of 3D-printed polylactide (PLA) artificial brood cells (capacity: 0.6 ml). (B) Artificial brood cells with L4 larvae inside a 24-well clear flat bottom plate, and (C) L4 feeding on medium. More about this image found in In vitro rearing of L4 larvae. (A) Design of 3D-printed polylactide (PLA)...

Fig. 1. Explosive growth of dorsal and ventral telencephalic domains. (A-C) Immunohistochemical staining for PCNA (green) and HuC/D (magenta) in combination with a nuclear stain (DAPI, blue) in coronal brain sections. Three levels of the anterior-posterior axis – anterior-mid-posterior – are sho... More about this image found in Explosive growth of dorsal and ventral telencephalic domains. (A-C) Immuno...

Fig. 2. EdU tracing reveals a neuronal stacking process during telencephalic growth. (A) Design of the EdU birth-dating experiment. Each experimental group is exposed to 4 mM EdU for 16 h (black boxes) at different time points during development. EdU is incorporated into the DNA of dividing cell... More about this image found in EdU tracing reveals a neuronal stacking process during telencephalic growth...

Fig. 3. Different pallial and subpallial growth dynamics identified by EdU tracing. (A,B) The absolute number of EdU + cells was quantified in 6-week-old coronal sections for both pallial (A) and subpallial (B) regions. Sections spanning the anterior-posterior axis were categorized into three l... More about this image found in Different pallial and subpallial growth dynamics identified by EdU tracing....

Fig. 4. Radial glia patterns in the developing telencephalon. (A) Immunohistochemical staining of GS (turquoise) on a 5 dph telencephalon section at a posterior level. Isolated GS + RGs are positioned one by one at the pallial surface (turquoise arrowheads) and in a dense cluster at the subpall... More about this image found in Radial glia patterns in the developing telencephalon. (A) Immunohistochemi...

Fig. 5. Distribution of the non-glial and neuroepithelial-like progenitors. (A-C) HCR-FISH targeting NGPmix (STMN1A+HMGB2A, NGPs, turquoise) and ZIC2 (NE-RGs, yellow) in combination with immunohistochemical staining for the progenitor marker SOX2 (magenta) on coronal sections along the anterior-... More about this image found in Distribution of the non-glial and neuroepithelial-like progenitors. (A-C) ...

Fig. 6. Progenitor heterogeneity in the developing telencephalon. (A,B) Adjacent coronal sections of the telencephalon at 5 dph along the anterior-posterior axis. The level of each section is indicated with a red line on the top-view brain illustration in the upper-left corner of each panel in A... More about this image found in Progenitor heterogeneity in the developing telencephalon. (A,B) Adjacent c...

Fig. 7. Intermediate progenitors in the killifish pallium. (A) HCR-FISH targeting CX43 (Astro-RGs, yellow), NGPmix (turquoise) and the immature excitatory neuron marker EOMESA (magenta) in combination with the nuclear stain DAPI (white) on a coronal section of the telencephalon at 5 dph. The ant... More about this image found in Intermediate progenitors in the killifish pallium. (A) HCR-FISH targeting ...

Fig. 8. Excitatory and inhibitory neurons in the 5   dph telencephalon. (A-C) Coronal sections of the telencephalon at 5 dph along the anterior-posterior axis. HCR-FISH targeting DLX1 (magenta) and GAD1B (turquoise) mRNA expression shows the distribution of immature and mature inhibitory neurons... More about this image found in Excitatory and inhibitory neurons in the 5   dph telencephalon. (A-C) Coro...

Fig. 1. Study site locations at (A) Guanaba Creek (Guanaba Indigenous Protected Area) and (B) Boy-Ull Creek (Springbrook National Park), southeast Queensland, Australia. Sampling was conducted over four periods from October 2022 to March 2023. At each site, independent biological replicates incl... More about this image found in Study site locations at (A) Guanaba Creek (Guanaba Indigenous Protected Are...

Fig. 2. Deployment of UVB dosimeters in larval amphibian habitats at Guanaba Creek (Guanaba Indigenous Protected Area) and Boy-Ull Creek (Springbrook National Park). Dosimeters ( n =3 per pool, total n =12 per site, n =24 across both sites) were placed in aquatic mounts floating at the water s... More about this image found in Deployment of UVB dosimeters in larval amphibian habitats at Guanaba Creek ...

Fig. 3. Deployment of UV and temperature sensors at study sites in (A) Guanaba Creek (Guanaba Indigenous Protected Area, n =7–13 UV sensors, n =12 temperature sensors) and (B) Boy-Ull Creek (Springbrook National Park, n =7–13 UV sensors, n =12 temperature sensors). Sensors were placed at 1 c... More about this image found in Deployment of UV and temperature sensors at study sites in (A) Guanaba Cree...

Fig. 4. Representative sampled UVI readings every 15 s for 24 h on January 20, 2023, at one site (Guanaba Creek; Guanaba Indigenous Protected Area) ( n =4 UV sensors) compared with the estimated clear sky UVI as predicted by the solar radiative transfer model (black line). Each sensor represents... More about this image found in Representative sampled UVI readings every 15 s for 24 h on January 20, 2023...