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Keywords: mCherry
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Journal Articles
Michael Brauchle, Simon Hansen, Emmanuel Caussinus, Anna Lenard, Amanda Ochoa-Espinosa, Oliver Scholz, Simon G. Sprecher, Andreas Plückthun, Markus Affolter
Journal:
Biology Open
Biol Open (2014) 3 (12): 1252–1261.
Published: 21 November 2014
... level. DARPins binding to GFP or mCherry were selected by ribosome display. For GFP, binders with K D as low as 160 pM were obtained, while for mCherry the best affinity was 6 nM. We then verified in cell culture their specific binding in a complex cellular environment and found an affinity cut-off...
Includes: Supplementary data