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Keywords: jGCaMP7s
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Journal Articles

Visualization of the biphasic calcium wave during fertilization in Caenorhabditis elegans using a genetically encoded calcium indicator

Katie M. Toperzer, Savannah J. Brennan, David J. Carroll, Eric A. Guisbert, Karen S. Kim Guisbert
Journal: Biology Open
Biol Open (2023) 12 (9): bio059832.
https://doi.org/10.1242/bio.059832
Published: 21 August 2023
...-egg fusion, we adapted the genetically encoded calcium indicator jGCaMP7s to visualize the moment of fertilization in Caenorhabditis elegans using fluorescence. We termed this tool the ‘CaFE’ reporter, for ‘calcium during fertilization in C. elegans ’. The CaFE reporter produced a robust signal...
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Includes: Supplementary data