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Keywords: Zebrafish
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Journal Articles
Biol Open (2021) 10 (3): bio058542.
Published: 09 March 2021
... zebrafish cardiovascular mutants, we identified a mutation in the prkd2 gene. Homozygous mutant embryos develop as wild type up to 36 h post-fertilization and initiate blood flow, but fail to maintain it, resulting in a complete outflow tract stenosis. We identified a mutation in the prkd2 gene that results...
Includes: Supplementary data
Journal Articles
Biol Open (2021) 10 (3): bio056382.
Published: 08 March 2021
...Rui Guo; Fei Li; Minxia Lu; Kangkang Ge; Lin Gan; Donglai Sheng ABSTRACT LIM homeobox 9 (Lhx9) is a member of the LIM homeodomain transcription factor family, which expresses and functions in various vertebrate tissues, such as the gonads and pineal gland. Previous studies on lhx9 in zebrafish have...
Includes: Supplementary data
Journal Articles
Biol Open (2021) 10 (2): bio050096.
Published: 25 February 2021
... zebrafish and mouse wound healing. Moreover, reduction at Tspan18 level resulted in aberrant vascular patterning, impaired vessel stability and defective arterial–venous specification. Tspan18 deficiency reduced VEGF, VEGFR2, Notch3 and EphrinB2, and increased EphB4, VEGFR3, Semaphorin3, Neuropilin and...
Includes: Supplementary data
Journal Articles
Biol Open (2020) 9 (11): bio053785.
Published: 06 November 2020
... indicate that miR-204 overexpression accelerates the development of BRAFV600E-driven melanoma. miniCoopR-I allows study of the impact that coding and non-coding modulators of pigmentation exert on melanomagenesis in adult zebrafish, uncoupling it from the impact that they exert on melanogenesis during...
Includes: Supplementary data
Journal Articles
Biol Open (2020) 9 (8): bio053363.
Published: 28 August 2020
... clinically would potentially hasten availability to DMD patients. In our study, we used dystrophin-deficient sapje and sapje-like zebrafish models of DMD for rapid and easy screening of several classes of serotonin pathway modulators as potential therapeutics. None of the candidate drugs tested significantly...
Journal Articles
Biol Open (2020) 9 (8): bio049304.
Published: 14 August 2020
... carbohydrates. In this study, we examined the physiological effects of human milk-derived opioid peptides, β-casomorphins (BCM), and compared them with bovine-milk-derived opioid peptides on pancreatic hormone regulation and β-cell regeneration. Exposure of wild-type zebrafish embryos to 50 µg/ml of human BCM-5...
Journal Articles
Biol Open (2020) 9 (7): bio047779.
Published: 21 July 2020
... tested whether elevated temperature extends the visual detection distance – the distance from which a visual stimulus is detected. To test this hypothesis, we investigated the effect of temperature on the visual detection distance of zebrafish ( Danio rerio ) larvae by measuring the largest distance from...
Includes: Supplementary data
Journal Articles
Biol Open (2020) 9 (5): bio049239.
Published: 07 May 2020
... developing inflammatory brain lesions. We used an RNASET2-deficient zebrafish model to elucidate the underlying disease mechanisms. Mutant and wild-type zebrafish larvae brain development between 2 and 5 days post fertilization (dpf) was examined by confocal live imaging in fluorescent reporter lines of the...
Includes: Supplementary data
Journal Articles
Biol Open (2020) 9 (5): bio050260.
Published: 04 May 2020
... incompletely understood. Due to their suitability for live imaging and their amenability to genetic and pharmacological manipulation, larval zebrafish provide an ideal platform for studying the regulation of secondary cell death in vivo . Here, we use RNA-seq gene expression profiling and compound screening to...
Includes: Supplementary data
Journal Articles
Biol Open (2020) 9 (4): bio051367.
Published: 13 April 2020
... have been previously associated with human disease. To understand the mechanisms by which GABRA1 regulates brain development, we developed a zebrafish model of gabra1 deficiency. gabra1 expression is restricted to the nervous system and behavioral analysis of morpholino injected larvae suggests that...
Includes: Supplementary data
Journal Articles
Biol Open (2019) 8 (12): bio046474.
Published: 09 December 2019
... types of cardiomyopathy. Here, we employed a zebrafish model to investigate the genetic replenishment strategies of using conditional and inducible promoters to rescue the deficiencies in the heart. tnnt2a mutations were induced in zebrafish via the CRISPR/Cas9 technique, and the mutants displayed heart...
Includes: Supplementary data
Journal Articles
Biol Open (2019) 8 (10): bio047829.
Published: 17 October 2019
... or adulthood. We discovered that switching on activated β-catenin in a subset of larval hepatocytes was sufficient to drive HCC initiation. To determine the role of Wnt/β-catenin signaling heterogeneity later in hepatocarcinogenesis, we performed RNA-seq analysis of zebrafish β-catenin-driven HCC. At...
Includes: Supplementary data
Journal Articles
Biol Open (2019) 8 (10): bio044347.
Published: 15 October 2019
... ) using morpholinos can rescue DA neurons in the PTEN-induced putative kinase 1 ( pink1 ) −/− zebrafish model of PD. In this article, we show results from our studies in mcu knockout zebrafish, which was generated using the CRISPR/Cas9 system. Functional assays confirmed impaired mitochondrial calcium...
Journal Articles
Biol Open (2019) 8 (9): bio044867.
Published: 18 September 2019
... effective treatments for patients affected by NM, we conducted a large scale chemical screen in a zebrafish model of NEB -related NM and an N-ethyl-N-nitrosourea (ENU)-based genetic screen in a mouse model of NEB exon 55 deletion, the most common NEB mutation in NM patients. Neither screen was able to...
Journal Articles
Biol Open (2019) 8 (9): bio039834.
Published: 09 September 2019
... growth factors (FGFs) direct migration, proliferation and survival of cranial neural crest cells (CNCCs) forming the human face. In this study, we analyzed bone and cartilage formation in the head of five dpf fgf8a ti282 zebrafish larvae and assessed gene expression levels for 11 genes involved in these...
Journal Articles
Biol Open (2019) 8 (5): bio042374.
Published: 24 May 2019
...Veronika Oralová; Joana T. Rosa; Mieke Soenens; Jan Willem Bek; Andy Willaert; Paul Eckhard Witten; Ann Huysseune ABSTRACT Zebrafish is now widely used in biomedical research as a model for human diseases, but the relevance of the model depends on a rigorous analysis of the phenotypes obtained...
Journal Articles
Biol Open (2019) 8 (5): bio043257.
Published: 13 May 2019
... combination. However, patient prognosis remains very poor, prompting the search for new models for drug discovery and testing, especially those that allow assessment of in vivo responses to treatment. Zebrafish xenograft models have an enormous potential to study tumor behavior, proliferation and cellular...
Includes: Supplementary data
Journal Articles
Biol Open (2019) 8 (5): bio040840.
Published: 01 May 2019
... relationship in bone development, we used a zebrafish larvae model to investigate how disruption of one of these signals affects the function of the other and impacts osteoblast differentiation. Our results showed that activation of Hh signaling negatively regulated autophagy. However, suppression of autophagy...
Journal Articles
Biol Open (2019) 8 (4): bio039768.
Published: 18 April 2019
... fertilized zebrafish ( Danio rerio ) eggs. We injected Texas Red dextran to trace vascular development. Embryos injected with the solvent of PGE2 served as vehicle. Distinct developmental changes were noted from 28–96 h post fertilization (hpf), showing an increase in embryonic tail flicks, pigmentation...
Includes: Supplementary data
Journal Articles
Biol Open (2019) 8 (3): bio040253.
Published: 04 March 2019
... in zebrafish using puromycin aminonucleoside (PAN) via treatment in the fish water to allow for further drug testing to develop anti-proteinuric drugs for the treatment of glomerular diseases. We noticed that fish that carry the nacre-mutation show a significantly higher susceptibility for the...