.... A pre-screening of strongly associated myopia-risk genes identified through human genome-wide association studies implicates efemp1 in myopia development, but how this gene impacts ocular growth remains unclear. Here, we modify efemp1 expression specifically in the retina of zebrafish. We found...

... in such a highly specific brain abnormality. To identify the shared consequence of JBTS gene dysfunction, we carried out transcriptomic analysis using zebrafish mutants for the JBTS-causative genes cc2d2a w38 , cep290 fh297 , inpp5e zh506 , talpid3 i264 and togaram1 zh510 and the Bardet-Biedl syndrome-causative...

... in permanent sensory deficits. Aquatic vertebrates like zebrafish ( Danio rerio ) have a specialized class of mechanosensory hair cells found in the lateral line system, allowing them to sense changes in water current. Unlike mammalian inner ear hair cells, lateral line hair cells can robustly regenerate...

... ABSTRACT Contemporary cardiac injury models in zebrafish larvae include cryoinjury, laser ablation, pharmacological treatment and cardiac dysfunction mutations. Although effective in damaging cardiomyocytes, these models lack the important element of myocardial hypoxia, which induces critical molecular...

.... We applied this approach to devise a method for the instantaneous determination of a zebrafish's genotype simply by examining its color. When a zebrafish mutant line must be propagated as heterozygotes due to homozygous lethality, employing this method allows facile identification of a population...

... zebrafish R::RT1 models were hampered by embryonic lethality and low penetrance of the malignant phenotype. Here, we overcome this by developing an adult zebrafish model in which the human R::RT1 isoform 9a is co-expressed with the frequently co-occurring oncogenic NRAS G12D mutation in haematopoietic stem...

...Eva E. Stefanova; Julian Vincent T. Dychiao; Mavis C. Chinn; Matin Borhani; Angela L. Scott ABSTRACT In contrast to mammals, zebrafish undergo successful neural regeneration following spinal cord injury. Spinal cord ependymo-radial glia (ERG) undergo injury-induced proliferation and neuronal...

...Laura Dorner; Benedikt Stratmann; Laura Bader; Marco Podobnik; Uwe Irion ABSTRACT The zebrafish ( Danio rerio ) is an important model organism for basic as well as applied bio-medical research. One main advantage is its genetic tractability, which was greatly enhanced by the introduction...

... exposure cessation. Using a developmental exposure protocol whereby zebrafish embryos were exposed to 0.5 and 1.5 mM iAs from 4–120 hours post fertilization (hpf) and then removed, we investigated the sustained effects of iAs on gene expression in the liver, survival, reproductive success...

...Vanessa Chong-Morrison; Sarah Mayes; Filipa C. Simões; Upeka Senanayake; Dervla S. Carroll; Paul R. Riley; Stephen W. Wilson; Tatjana Sauka-Spengler ABSTRACT Due to its genetic amenability coupled with advances in genome editing, zebrafish is an excellent model to examine the function of (epi...

..., and the resultant protein interacts with FGFR1 in the FGF signaling pathway during development. Studies on flrt2 have focused mainly on its roles in the brain, heart and chondrogenesis. However, reports on its expression and function in the zebrafish retina are lacking. Here, we detected the high expression...

... precursor cells of the zebrafish anterior hindbrain . Gene Expr. Patterns 5 , 35 - 41 . 10.1016/j.modgep.2004.06.009 Ahmed , M. and Streit , A. ( 2018 ). Lsd1 interacts with cMyb to demethylate repressive histone marks and maintain inner ear progenitor identity . Development...

... in the process, but the underlying biology is still poorly understood. This is at least partially being explained by complexity and costs in using mammalian aging models. To understand aging-related vascular biology of impaired wound healing, we used zebrafish and turquoise killifish fin regeneration models...

... models. Therefore, we took the approach of determining the effects of VPA treatment on zebrafish OT development as a first step in identifying the mechanisms that allow its formation. We describe normal OT development during the first 5 days of development and show that in VPA-treated embryos, neuronal...

... a common mechanism operates in these RREs. We identified three RREs from the zebrafish fn1b promoter by searching for conserved sequences within the surrounding genomic regions of regeneration-induced genes and performed a transgenic assay for regeneration response. Two regions contained in the transposons...

..., confocal imaging of fluorescent cancer cells and their response to treatments in zebrafish larvae yields valuable information. While zebrafish larvae are well suited for confocal imaging, the lack of efficient processing of large datasets remains a severe bottleneck. To alleviate this problem, we present...

... exposed zebrafish ( Danio rerio ) embryos to either chemical cues from crushed embryos (simulating egg predation) or to blank water control. Embryos exposed to alarm cues hatched sooner, and had shorter body lengths and underdeveloped fins, relative to larvae from the water treatment. Burst swimming speed...

... in isolated zebrafish in which lateral line hair cells have been chemically ablated. These results suggest that modulation of pth2 by social context is acutely dependent on mechanosensory information from the lateral line. However, it is unclear how a congenital loss of lateral line function influences...

... bold, whereas animals with the opposite behavioral pattern are shy. Here, we determined whether this bimodal characterization of bold versus shy adequately captures the breadth of behavioral variation in zebrafish or if there are more than these two subtypes. To identify behavioral categories, we...

...Simon M. Hughes; Roberta C. Escaleira; Kees Wanders; Jana Koth; David G. Wilkinson; Qiling Xu ABSTRACT To address questions of stem cell diversity during skeletal myogenesis, a Brainbow-like genetic cell lineage tracing method, dubbed Musclebow2 , was derived by enhancer trapping in zebrafish...