...Martha S. C. Xelhuantzi; Daniel Ghete; Amy Milburn; Savvas Ioannou; Phoebe Mudd; Grant Calder; José Ramos; Peter J. O'Toole; Paul G. Genever; Chris MacDonald ABSTRACT Although some budding yeasts have proved tractable and intensely studied models, others are more recalcitrant. Debaryomyces hansenii...

...Haiqiong Chen; Rinse de Boer; Arjen M. Krikken; Fei Wu; Ida van der Klei ABSTRACT Pex23 family proteins localize to the endoplasmic reticulum and play a role in peroxisome and lipid body formation. The yeast Hansenula polymorpha contains four members: Pex23, Pex24, Pex29 and Pex32. We previously...

...Channarong Nasalingkhan; Naraporn Sirinonthanawech; Chalongrat Noree ABSTRACT As part of our studies of yeast aldehyde dehydrogenase (Ald4p) assembly, we identified a population of transformants (SWORD strain) that show more robust filament formation of GFP-tagged Ald4p (Ald4p-GFP) than...

...Sarah H. Carl; Lea Duempelmann; Yukiko Shimada; Marc Bühler ABSTRACT Adenine auxotrophy is a commonly used non-selective genetic marker in yeast research. It allows investigators to easily visualize and quantify various genetic and epigenetic events by simply reading out colony color. However...

... assembly/disassembly in vivo . Here, we demonstrate that addition of exogenous acetaldehyde can quickly induce the supramolecular assembly of MTS-deleted aldehyde dehydrogenase Ald4p in yeast cytoplasm. Also, by using PCR-based modification of the yeast genome, cytoplasmically targeted Ald4p cannot...

...Oliver Glomb; Lara Bareis; Nils Johnsson ABSTRACT The polarisome comprises a network of proteins that organizes polar growth in yeast and filamentous fungi. The yeast formin Bni1 and the actin nucleation-promoting factor Bud6 are subunits of the polarisome that together catalyze the formation...

... of the sensing of hyperosmotic shock, the signaling cascades which mediate the cells' responses, and the mechanisms of adaptation to hyperosmotic conditions via synthesis and retention of osmolytes, namely glycerol, in yeast [reviewed in Saito and Posas (2012) ]. However, the immediate consequences...

... and reproduction in any medium provided that the original work is properly attributed. Summary: In yeast mitochondria the Gcn5 protein is present in the mitoplasts and is localized in the inner mitochondrial membrane. Its deletion affects the mitochondrial phenotype and is related to a marked decrease...

... in human. Here, we generate a yeast strain expressing F265L tubulin mutant as the sole source of β-tubulin. The F265L mutation does not preclude expression of a stable β-tubulin protein which is incorporated into microtubules. However, impaired cell growth was observed at high temperatures along...

... was localized to the electron-dense filamentous structures in mitochondria. These results together showed that a major component of MFIBs is Ald4p. In addition, we demonstrate that MFIBs are common features that appear in mitochondria of many species of yeast. § Author for correspondence ( miyakawa...