... the interconnection between CK and cell junctions using membrane β-catenin as a marker during testicular development in the Xenopus tropicalis model. Immunohistochemistry on juvenile (5 months old) testes revealed co-expression of CK, membrane β-catenin and E-cadherin. Adult (3-year-old males) samples confirmed only...

..., is indispensable for amphibian metamorphosis. However, specific roles for the TRβ isoform in metamorphosis are poorly understood. To address this issue, we generated trβ- disrupted Xenopus tropicalis tadpoles using the CRISPR-Cas system . We first established a highly efficient and rapid workflow for gene...

...Tereza Tlapakova; Thi Minh Xuan Nguyen; Marketa Vegrichtova; Monika Sidova; Karolina Strnadova; Monika Blahova; Vladimir Krylov ABSTRACT The origin of somatic cell lineages during testicular development is controversial in mammals. Employing basal amphibian tetrapod Xenopus tropicalis we...

...Naoko Goto-Inoue; Akihiko Kashiwagi; Keiko Kashiwagi; Tsukasa Mori ABSTRACT In developmental and cell biology it is crucial to evaluate the dynamic profiles of metabolites. An emerging frog model system using Xenopus tropicalis, whose genome sequence and inbred strains are available, is now ready...

... mutagenic activity in F0 frogs could result in developmental disorders or sterility, which would reduce the number of F1 progeny and make F1 phenotypical analysis difficult. We used the 3′ untranslated region of DEADSouth gene (DS-3′) of Xenopus tropicalis to solve this problem, because the addition...

... a transcription activator-like effector, and the non-specific nuclease domain of FokI. Using Xenopus tropicalis ( X. tropicalis ), we compared the toxicities and somatic mutation activities of four TALEN architectures in a side-by-side manner: a basic TALEN, a scaffold with the same truncated N- and C-terminal...