...Keisuke Nakajima; Yoshio Yaoita ABSTRACT Zinc-finger nucleases, transcription activator-like effector nucleases (TALENs) and the CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) system are potentially powerful tools for producing tailor-made knockout...

... as manipulating DNA in test tubes, which is made possible by two recently developed techniques based on either the customizable DNA binding protein, TALEN, or the CRISPR/Cas9 system. Here, we describe a series of efficient applications derived from these two technologies, in combination with various homologous...

...Ken-ichi T. Suzuki; Yukiko Isoyama; Keiko Kashiwagi; Tetsushi Sakuma; Hiroshi Ochiai; Naoaki Sakamoto; Nobuaki Furuno; Akihiko Kashiwagi; Takashi Yamamoto Summary Recently, gene editing with transcription activator-like effector nucleases (TALENs) has been used in the life sciences. TALENs can...

... (TALENs) can induce a targeted DNA double-strand break at the specific target genomic locus, leading to the frameshift-mediated gene disruption. However, the assays for their activity on the endogenous genomic loci remain limited. Herein, we describe a versatile modified lacZ assay to detect frameshifts...