... poorly understood. CD133/ Prom1 expression has been used as a marker to identify adult stem cell populations in various organs and often associated with cancer cells that have stem-like properties. Using an antibody targeted to CD133 and a Cre recombinase-based lineage tracing strategy, we found...

...Eric H. Lee; Daniel Zinshteyn; Fred Miglo; Melissa Q. Wang; Jessica Reinach; Cindy M. Chau; Joseph M. Grosstephan; Iliana Correa; Kelly Costa; Alberto Vargas; Aminah Johnson; Sheila M. Longo; Jennifer I. Alexander; Alana M. O'Reilly ABSTRACT Stem cells cycle between periods of quiescence...

... disruption of one copy of the CHD8 gene in the E11 hESC line. The E11 clone expressed the pluripotent stem cell marker Oct4 in virtually all cells, similar to wt hESCs ( Fig. 2 A,B; Fig. S2A ) indicating that mono-allelic disruption of CHD8 has not compromised the pluripotent state of these cells. We...

...Simon M. Hughes; Roberta C. Escaleira; Kees Wanders; Jana Koth; David G. Wilkinson; Qiling Xu ABSTRACT To address questions of stem cell diversity during skeletal myogenesis, a Brainbow-like genetic cell lineage tracing method, dubbed Musclebow2 , was derived by enhancer trapping in zebrafish...

...Viju Vijayan Pillai; Prasanthi P. Koganti; Tiffany G. Kei; Shailesh Gurung; W. Ronald Butler; Vimal Selvaraj ABSTRACT Although derivation of naïve bovine embryonic stem cells is unachieved, the possibility for generation of bovine induced pluripotent stem cells (biPSCs) has been generally reported...

..., but its developmental roles in roots have been largely overlooked. Here, we show that plastid translation is connected to the stem cell patterning in lateral root primordia. The RFC3 gene encodes a plastid-localized protein that is a conserved bacterial ribosomal protein S6 of β/γ proteobacterial origin...

...Ingrid R. Cordeiro; Daiana V. Lopes; José G. Abreu; Katia Carneiro; Maria I. D. Rossi; José M. Brito ABSTRACT Human adipose-derived stromal cells (hADSC) are a heterogeneous cell population that contains adult multipotent stem cells. Although it is well established that hADSC have skeletal...

.... Stunnenberg; Lucia Altucci Summary Exploitation of embryonic stem cells (ESC) for therapeutic use and biomedical applications is severely hampered by the risk of teratocarcinoma formation. Here, we performed a screen of selected epi-modulating compounds and demonstrate that a transient exposure of mouse ESC...

...Heikki Kiiski; Riikka Äänismaa; Jyrki Tenhunen; Sanna Hagman; Laura Ylä-Outinen; Antti Aho; Arvi Yli-Hankala; Stepani Bendel; Heli Skottman; Susanna Narkilahti Summary The possibilities of human pluripotent stem cell-derived neural cells from the basic research tool to a treatment option...

... conditions. The nematode pten homologue daf-18 is essential for maintenance of survival and germline stem cell quiescence during this period ( Fukuyama et al., 2006 ; Sigmond et al., 2008 ), but the details of the signaling network(s) in which it functions remain to be elucidated. Here, we report...

...Marta E. Kalamarz; Indira Paddibhatla; Christina Nadar; Shubha Govind Summary How cell-intrinsic regulation of the cell cycle and the extrinsic influence of the niche converge to provide proliferative quiescence, safeguard tissue integrity, and provide avenues to stop stem cells from giving rise...