... their phenotypes. We show that hasp-1 is responsible for all predicted functions of Haspin and that loss of function of hasp-1 using classical and conditional alleles produces defects in germline stem cell proliferation, spermatogenesis, and confirms its role in oocyte meiosis. Genetic analysis suggests hasp-1...

... the RA signaling pathway. Furthermore, we suggest that NANOS3-mediated inhibition of spermatogonial differentiation is important to initiate synchronized spermatogenesis from seminiferous stages VII-VIII, and indirectly helps to maintain correct testicular germ cell associations. Further molecular...

...- (immature) or 7-week-old (mature) Etv5 −/− mice. Partial restoration of germ cell layers in the seminiferous tubules and spermatogenesis was observed in all immature testes but not in mature adult testes at 2 months post-transplantation. The presence of heterologous genes Etv5 and EGFP in recipient...

.... mulet encodes Tubulin-binding cofactor E-like (TBCEL), suggesting a role for microtubule dynamics in individualization. Indeed, a population of ∼100 cytoplasmic microtubules fails to disappear in mulet mutant testes during spermatogenesis. This persistence, detected using epi-fluorescence and electron...

... and one long non-coding RNA (lncRNA) in mouse testis during spermatogenesis, indicating their tightly controlled synthesis. Additionally, lncRNA transcripts are increased by exposure to oxidative stress in mouse GC-1 germ cell line. STK35 knockout (KO) mice lacking all three RNAs are born at sub-Mendelian...

... the scat 1 mutant to be male sterile in Drosophila with individualization problems occurring during spermatogenesis. Another typically observed phenotype was the abnormal nuclear structure in elongated mutant cysts. When examining the structure and function of the Golgi, a failure in acrosome formation...

... and regulates germline development in hermaphrodite worms. Specifically, the normal transition from spermatogenesis to oogenesis in the hermaphrodite germline fails in ife-3 mutants. This failure to switch is reversed by inhibiting expression of the key masculinizing gene, fem-3 , suggesting ife-3 resembles...

... a conditional deletion of BAZ1B. Although BAZ1B deletion causes ectopic γH2AX signals on synapsed autosomes during the early pachytene stage, BAZ1B is dispensable for fertility and critical events during spermatogenesis. BAZ1B deletion does not alter events on unsynapsed axes and pericentric heterochromatin...

... sperm into wild-type oocytes led to a block at the two-pronucleus to zygote transition, whereas normal preimplantation development and healthy pups were obtained through injection of miR-dKO round spermatids. Our data demonstrate that miR-34b/c and miR-449a/b/c are essential for normal spermatogenesis...

... permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Spermatogenic cycle Spermatogenesis Germ cell Mouse Agent-based model Simulation The spermatogenic cycle delineates the periodic development of male germ cells...

... Sperm individualization Spermatogenesis Tubulin post translation modifications Cilia are specialized cell organelles that have motility and sensory functions. Their axoneme is built through nucleation of MTs from a basal body anchored at the plasma membrane, while assembly of the remaining...

... of gastrulation. Functional studies of vasa and tudor reveal that these genes are not required for germ cell formation, but that vasa is required in adult males for spermatogenesis. Taken together, our results provide evidence that Oncopeltus germ cells may form in the absence of germ plasm, consistent...

... that tubulobulbar complexes internalize intercellular junctions and that they are a significant component of the sperm release mechanism. Fig. 5. Cross sections through apical Sertoli cell lobules containing mature spermatids at Stage VII of spermatogenesis in control (A) and cortactin siRNA treated (B,C...

..., initiation and maintenance of spermatogenesis. It was recently shown that many spermatocytes underwent apoptosis in the testes of Hsp90α KO mice. We had generated Hsp90α KO mice independently and confirmed this phenotype. However, the important question of whether Hsp90α is required to maintain...

...Katja Leser; Stephan Awe; Bridlin Barckmann; Renate Renkawitz-Pohl; Christina Rathke Summary By a conserved cellular differentiation process, spermatogenesis leads to formation of haploid sperm for successful reproduction. In Drosophila and in mammals, post-meiotic spermatid differentiation depends...