... reprogramming and concurrently investigated signaling relevant to pluripotency of the bovine blastocyst inner cell mass (ICM). First, we identified that forced expression of SV40 large T antigen together with the reprogramming genes ( OCT4 , SOX2 , KLF4 and MYC ) substantially enhanced the reprogramming...

...Yoshikazu Haramoto; Mino Sakata; Shin Kobayashi ABSTRACT X chromosome inactivation (XCI), determined during development, remains stable after embryonic cell divisions. However, primordial germ cells (PGCs) are exceptions in that XCI is reprogrammed and inactivated X chromosomes are reactivated...

...-based therapy. However, its wide adoption is precluded by several challenges including low reprogramming efficiency and potential for malignant transformation. Better understanding of the molecular mechanisms of the changes that cells undergo during reprograming is needed to improve iPSCs generation...

... and LIF signaling for pluripotency establishment in reprogramming is unclear. We screened the marker genes critical for complete reprogramming during mouse iPSC generation, and identified genes including Esrrb that are responsive to LIF/Jak pathway signaling. Overexpression of Esrrb resumes...

... potent reprogramming characteristics will provide opportunities for the use of cells with specific genotypes to study the pathogenesis and molecular mechanisms of disease in vitro . Urine-derived cells reprogrammed by lentiviral transduction of pre-defined transcription factors is a demonstration...

... of SCNT embryos at the two-cell stage revealed that the treatment with TSA and VC resulted in the upregulated expression of previously identified reprogramming-resistant genes. Moreover, the expression of early-embryo-specific retroelements was upregulated by the TSA and VC treatment. The enhanced gene...

...Sumitha Prameela Bharathan; Kannan Vrindavan Manian; Syed Mohammed Musheer Aalam; Dhavapriya Palani; Prashant Ajit Deshpande; Mankuzhy Damodaran Pratheesh; Alok Srivastava; Shaji Ramachandran Velayudhan ABSTRACT Low efficiency of somatic cell reprogramming and heterogeneity among human induced...

...Sharan Paul; Lance Pflieger; Warunee Dansithong; Karla P. Figueroa; Fuying Gao; Giovanni Coppola; Stefan M. Pulst ABSTRACT We developed an adenoviral vector, in which Yamanaka's four reprogramming factors (RFs) were controlled by individual CMV promoters in a single cassette (Ad-SOcMK...

... . 10.1091/mbc.E06-09-0872 Efe , J. A. , Hilcove , S. , Kim , J. , Zhou , H. , Ouyang , K. , Wang , G. , Chen , J. and Ding , S. ( 2011 ). Conversion of mouse fibroblasts into cardiomyocytes using a direct reprogramming strategy . Nat. Cell Biol. 13 , 215 - 222...

..., senescence, premature aging and associated diseases. Induced pluripotent stem (iPS) cells have been successfully generated from various types of cells and used to model human diseases. It remains unclear whether levels of lamin A influence reprogramming of somatic cells to pluripotent states during iPS...