... to guide cell differentiation and fate decisions. The Srrm2 splicing factor has recently been implicated in developmental disorders and diseases, but its role in early mammalian development remains unexplored. Here, we show that Srrm2 dosage is critical for maintaining embryonic stem cell pluripotency...

... Review explores cutting-edge research and its translational potential discussed at the 16th European Summer School on Stem Cell Biology and Regenerative Medicine (Spetses, Greece, 12th–19th September 2023). Pluripotency Regenerative medicine Stem cell therapies Stem cells Summer school...

... bodies. PLPP3-deficient ESC strongly decrease the amount of FOXA2-expressing cells and fail to properly downregulate the expression of pluripotency factors when subjected to an endoderm-directed differentiation protocol. Impaired endoderm differentiation correlated with a transient reduction in nuclear...

...Yao Xiao; Froylan Sosa; Pablo J. Ross; Kenneth E. Diffenderfer; Peter J. Hansen ABSTRACT Bovine embryonic stem cells (ESC) have features associated with the primed pluripotent state including low expression of one of the core pluripotency transcription factors, NANOG. It has been reported...

...Viju Vijayan Pillai; Prasanthi P. Koganti; Tiffany G. Kei; Shailesh Gurung; W. Ronald Butler; Vimal Selvaraj ABSTRACT Although derivation of naïve bovine embryonic stem cells is unachieved, the possibility for generation of bovine induced pluripotent stem cells (biPSCs) has been generally reported...

... to cell differentiation did not show a large change in expression. On the other hand, genes related to pluripotency, such as Oct4 and Sox2 , showed a twofold increase in expression upon application of 60 MPa hydrostatic pressure for 10 min. Although these changes did not persist after overnight culture...

...Antonio Barral; Isabel Rollan; Hector Sanchez-Iranzo; Wajid Jawaid; Claudio Badia-Careaga; Sergio Menchero; Manuel J. Gomez; Carlos Torroja; Fatima Sanchez-Cabo; Berthold Göttgens; Miguel Manzanares; Julio Sainz de Aja ABSTRACT Pluripotency is regulated by a network of transcription factors...

...Ade Kallas-Kivi; Annika Trei; Artjom Stepanjuk; Katrin Ruisu; Keiu Kask; Margus Pooga; Toivo Maimets ABSTRACT The maintenance of the pluripotency of human embryonic stem (hES) cells requires special conditions for culturing. These conditions include specific growth factors containing media...

...; Giovanna Lazzari; Poul Hyttel; Hendrik G. Stunnenberg; Martijn Huynen; Roger A. Pedersen; Andras Dinnyes ABSTRACT During early mammalian development, transient pools of pluripotent cells emerge that can be immortalised upon stem cell derivation. The pluripotent state, ‘naïve’ or ‘primed’, depends...

... pluripotent stem cells (hiPSCs) demand extensive characterization of isolated clones before their use in downstream applications. By monitoring human fibroblasts undergoing reprogramming for their morphological changes and expression of fibroblast (CD13), pluripotency markers (SSEA-4 and TRA-1-60...

... in the realm of Stat3, self-renewal signaling, and pluripotent stem cell biology. Ultimately, this finding may facilitate the development of conditions for the establishment of authentic non-rodent ESCs. ‡ These authors contributed equally to this work § Author for correspondence ( qying...

... of loss of pluripotency and Activin/Nodal, BMP4 and Wnt signalling in fate assignment during the early stages of differentiation of mouse ES cells in culture. During the initial period of differentiation, cells exit from pluripotency and enter an Epi-like state. Following this transient stage, and under...

.... To this end, pluripotent mouse Embryonic Stem (mES) cells have proven to be a useful model system. However, understanding how transcription factors and signalling pathways affect decisions made by individual cells is confounded by the fact that measurements are generally made on groups of cells, whilst...

... Afanassieff Summary Not much is known about the molecular and functional features of pluripotent stem cells (PSCs) in rabbits. To address this, we derived and characterized 2 types of rabbit PSCs from the same breed of New Zealand White rabbits: 4 lines of embryonic stem cells (rbESCs), and 3 lines of induced...

..., senescence, premature aging and associated diseases. Induced pluripotent stem (iPS) cells have been successfully generated from various types of cells and used to model human diseases. It remains unclear whether levels of lamin A influence reprogramming of somatic cells to pluripotent states during iPS...