...Carmen Daems; El-Sayed Baz; Rudi D'Hooge; Zsuzsanna Callaerts-Végh; Patrick Callaerts ABSTRACT Mutations in the highly conserved Pax6 transcription factor have been implicated in neurodevelopmental disorders and behavioral abnormalities, yet the mechanistic basis of the latter remain poorly...

... and continuous production of neurons. The Pax6 transcription factor plays a major role in INM by regulating various downstream molecules. We have previously observed abnormal INM and unstable localization of the centrosome in APs of the Pax6 homozygous mutant rat embryo. To understand the mechanisms of INM, we...

... in Xenopus laevis embryos, we performed the targeted gene disruption of tyrosinase ( tyr ) and pax6 genes that are involved in pigmentation and eye formation, respectively. We constructed TALENs targeting tyr and pax6 and injected their mRNAs into fertilized eggs at the one-cell stage. Expectedly...