... density cultures ( Fig. 6 A and B). This suggests that DCA reduces glycolytic activity and increases OXPHOS in PC3 cells, both typical of the hallmark of Warburg effect ( Warburg, 1956 ). Fig. 6. Effect of dichloroacetate (DCA) addition on oxidative phosphorylation in PC3 cells. mRNA expression...

... respiratory chain complexes High-resolution clear native polyacrylamide gel electrophoresis In-gel activity assay Supercomplex Oxidative phosphorylation Enhanced chemiluminescence The in-gel activity assay (IGA) has been used to detect and simultaneously compare associations of mitochondrial...

... and impaired respiration, whereas genetic activation of Nrf2 increases the mitochondrial membrane potential and ATP levels, the rate of respiration and the efficiency of oxidative phosphorylation. We further show that Nrf2-deficient cells have increased production of ATP in glycolysis, which is then used...