...Federico Nebuloni; Joseph Morgan; Edmond J. Walsh; Peter R. Cook ABSTRACT Organoids grow in vitro to reproduce structures and functions of corresponding organs in vivo . As diffusion delivers nutrients over only ∼200 µm, refreshing flows through organoids are required to avoid necrosis...

...Shohei Yoshimoto; Masahide Taguchi; Satoko Sumi; Kyoko Oka; Kazuhiko Okamura ABSTRACT Three-dimensional (3D) cell culture models such as spheroids and organoids are widely used in the field of experimental biology. To analyze these 3D experimental models, formalin-fixed paraffin-embedded (FFPE...

... organoid models to understand brain and eye development, cell-fate regulation and disease pathophysiology ( Mahato et al., 2022 ; Sriram et al., 2020 ; Susaimanickam et al., 2017 ). Mimicking human neurological disorders in rodent models is often challenging due to the species-specific differences...

.... Here we describe a method for culturing human glioblastoma stem cells (GSCs) in 3D by co-culturing them with pluripotent stem cell-derived brain organoids. This requires multiple coordinated steps, including the generation of cerebral organoids, and the growth and fluorescence tagging of GSCs. We...

...Robin H. Powell; Michael S. Behnke ABSTRACT Recent years have seen significant developments in the ability to continuously propagate organoids derived from intestinal crypts. These advancements have been applied to mouse and human samples providing models for gastrointestinal tissue development...

... (SAG) for granule cell development. After 35 days, differentiation products exhibited similar structures and neuronal markers reported in prior ‘organoid’ and ‘spheroid’ protocols. This included cells positive for KIRREL2 (a marker of early cerebellar neuroepithelium) and ZIC1 (a marker for granule...