...Joshua Hawley; Robert Lea; Veronica Biga; Nancy Papalopulu; Cerys Manning ABSTRACT In the developing mouse ventral spinal cord, HES5, a transcription factor downstream of Notch signalling, is expressed as evenly spaced clusters of high HES5-expressing neural progenitor cells along the dorsoventral...

... to the modification of tRNA wobble uridines and the translational regulation of codon-biased genes. Here we characterize H2a.z , encoding the variant H2a histone H2A.Z, as an indirect Elongator target. We further show that canonical Notch signaling, a pathway directed by H2A.Z, is perturbed as a consequence of Elp1...

... of vessels to generate mature arborizing structures. The Notch pathway is essential for arterial–venous specification and the maturation of nascent vessels. We have determined that Tspan18, a member of the Tetraspanin family, is expressed in developing vessels but not in mature vasculature in zebrafish...

..., we first examine the expression of canonical stem cell markers in human RMS tumors and cell lines. We then describe a method to grow FP-RMS cell lines as rhabdospheres and demonstrate that these spheres are enriched in expression of canonical stemness factors as well as Notch signaling components...

... to a developing conceptus. Notch signaling utilizes a cell–cell contact dependent mechanism to drive cell behaviors, such as differentiation and invasion. In mice, Notch2 is needed for proper placentation and embryo survival. We used transgenic mice with a dominant-negative form of Mastermind-like1 and Cyp19-Cre...

...-like connective tissue are set up among intersegmental leg joint cells expressing odd-skipped genes, and that Notch signaling is necessary and locally sufficient to trigger stripe expression. This study also finds that odd-skipped genes and stripe are both required downstream of Notch to promote...

...Yunlong Li; Tong Liu; Junzheng Zhang ABSTRACT The evolutionarily conserved Notch signaling pathway plays crucial roles in various developmental contexts. Multiple mechanisms are involved in the regulation of Notch pathway activity. Identified through a genetic mosaic screen, we show that the ATPase...

...Silvia Gutnik; Yann Thomas; Yanwu Guo; Janosch Stoecklin; Anca Neagu; Lionel Pintard; Jorge Merlet; Rafal Ciosk ABSTRACT The Notch signalling pathway is a conserved and widespread signalling paradigm, and its misregulation has been implicated in numerous disorders, including cancer. The output...

... being determined. Here, we show that Fank1 and Jazf1 are necessary and sufficient for ciliated cell differentiation in the mouse airways. Cilia Lung Cell fate Foxj1 Mcin Notch The airway mucociliary escalator consists of secretory and multicilated cells and captures inhaled debris...

...Alexander M. Herman; Alexander M. Rhyner; W. Patrick Devine; Sean P. Marrelli; Benoit G. Bruneau; Joshua D. Wythe ABSTRACT Canonical Notch signaling requires the presence of a membrane bound ligand and a corresponding transmembrane Notch receptor. Receptor engagement induces multiple proteolytic...

...Zehua Wang; Jialan Lyu; Fang Wang; Chen Miao; Zi Nan; Jiayu Zhang; Yongmei Xi; Qi Zhou; Xiaohang Yang; Wanzhong Ge ABSTRACT The Notch signaling pathway is highly conserved across different animal species and plays crucial roles in development and physiology. Regulation of Notch signaling occurs...

... domain of Crb stabilises the Notch receptor and thereby ensures proper Notch signalling and specification of the correct number of neuroblasts. * Author for correspondence ( [email protected] ) Competing interests The authors declare no competing or financial interests. 6 12 2017 7...

...). The molecular dynamics that occur at the NSD boundary relative to adjacent OV cells is not well defined. The Tbx1 transcription factor gene expression pattern is complementary to the NSD, and inactivation results in expansion of the NSD and expression of the Notch ligand, Jag1 mapping, in part of the NSD...

...Sophie R. Miller; Surangi N. Perera; Clare V. H. Baker ABSTRACT Perivascular/mural cells originate from either the mesoderm or the cranial neural crest. Regardless of their origin, Notch signalling is necessary for their formation. Furthermore, in both chicken and mouse, constitutive Notch1...

... in response to leptin knockdown were determined by MTT assay and flow cytometry, respectively. The effect of leptin knockdown on the Notch and JAK/STAT3 signaling pathways was further examined by western blot. Leptin expression was significantly increased in NSCLC cell lines compared with normal human...

...Niurka Trujillo-Paredes; Concepción Valencia; Gilda Guerrero-Flores; Dulce-María Arzate; José-Manuel Baizabal; Magdalena Guerra-Crespo; Ayari Fuentes-Hernández; Iván Zea-Armenta; Luis Covarrubias ABSTRACT Notch signalling is a well-established pathway that regulates neurogenesis. However, little...

... can reverse transient imbalances of Notch signaling that result in defective organ proportions during repair. Our results reveal inextinguishable hair-cell regeneration in the lateral line, and suggest that the neuromast epithelium is formed by plastic territories that are maintained by continuous...

... was observed when the transgene was induced in the basal layer of the skin of adult mice leading to hair loss and open sores. The Ets1-mediated phenotype is accompanied by a variety of changes in gene expression including alterations in Notch signaling, a crucial mediator of normal skin differentiation...

... morphants were due to a derepression of rbpj mRNA. Rbpj protein is a key player of Notch signalling. Because segmentation involves complex cross-talk between several signalling pathways, we analysed how rbpj derepression impacted these pathways. We found that rbpj derepression stimulated the Notch pathway...

..., posteriorly-expressed Wnt1 promotes caudal and Delta expression early in development to generate a growth zone from which segments will later bud off. caudal maintains the undifferentiated growth zone by dampening Delta expression, and hence Notch-mediated segmentation occurs just outside the caudal domain...