... understood. Here, we study the effect upon mitochondria–lysosome MCSs of two GDAP1 clinical variants located in the α-loop interaction domain of the protein. p.Thr157Pro dominant variant causes the increase in these MCSs that correlates with a hyper-fissioned mitochondrial network. In contrast, p.Arg161His...

... for mitochondrial transcriptomics. The analyses show that a decrease of the Cox1 transcript correlates with reduced respiratory complex IV activity in older mice livers. Ageing Mitochondria Nanopore Transcriptomics Deutsche Forschungsgemeinschaft (DFG) EXC 2067/1- 390729940 DFG...

... regimes. As such, there is an urgent need to develop new antimicrobial agents that can combat these multidrug-resistant superbugs. Mitochondria are central regulators of metabolism and other cellular functions, including the regulation of innate immunity pathways involved in the defense against infection...

... tissue between wild-type and knockout mice. However, mitochondria isolated from brown fat of cd47 deficient mice had significantly higher rates of free fatty acid-mediated uncoupling. This suggests that enhanced fuel availability via white adipose tissue lipolysis may perpetuate elevated brown adipose...

.... The statistics showed in Fig. 7 E included 44, 204, 167, and 94 mitochondria of the control, CG5903/MIC26-MIC27 -, Mitofilin/MIC60 -, and QIL1/MIC13 -knockdown, respectively. Fly DNA was extracted by homogenizing about 50 flies in 200 μl of buffer containing 10 mM Tris (pH 8.0), 1 mM EDTA, 25 mM NaCl...

... pathologies by decreasing the rough eye phenotype, oxidative stress, apoptosis and neurodegeneration and increasing the lifespan, body weight, phototaxis and climbing activity in the Drosophila model of Alzheimer's disease. Miro Tau Aβ 42 Appl Mitochondria Alzheimer's disease...

...Susana Ponte; Lara Carvalho; Maria Gagliardi; Isabel Campos; Paulo J. Oliveira; António Jacinto ABSTRACT Mitochondria adapt to cellular needs by changes in morphology through fusion and fission events, referred to as mitochondrial dynamics. Mitochondrial function and morphology are intimately...

... to other organelles, whereas mitochondria generate energy in the form of ATP. Mitochondria and the ER form physical interactions, defined as mitochondria–ER contact sites (MERCs) to exchange metabolites such as calcium ions (Ca 2+ ) and lipids. Sites of contact between mitochondria and the ER can regulate...

... influx in mcu −/− zebrafish. We also used in vivo calcium imaging and fluorescent assays in purified mitochondria to investigate mitochondrial calcium dynamics in a pink1 −/− zebrafish model of PD. Mitochondrial morphology was evaluated in DA neurons and muscle fibers using immunolabeling and transgenic...

... homologue proposed as a mitochondrial biogenesis regulator, has minimal effects on the phenotype of tko 25t , considered a fly model for mitochondrial disease. Mitochondria Mitochondrial biogenesis Mitochondrial disease Transcriptional co-activator The PGC-1 coactivators are widely...

...Gigi Y. Lau; Nicholas Barts; Richard C. Hartley; Michael Tobler; Jeffrey G. Richards; Michael P. Murphy; Sabine Arndt ABSTRACT In this paper, we outline the use of a mitochondria-targeted ratiometric mass spectrometry probe, MitoA, to detect in vivo changes in mitochondrial hydrogen sulfide (H 2 S...

... CN5 deletion differently affects the growth of two strains. The defective mitochondrial phenotype is related to a marked decrease in mtDNA content, which also involves the deletion of specific regions of the molecule. We also show that in wild-type mitochondria the Gcn5 protein is present...

... of the anti-aging compounds rapamycin and AICAR on energy metabolism in these cells was determined. mTOR AMP kinase Aging Mitochondria Rapamycin Pyruvate addiction Several lines of evidence suggest the involvement of mitochondrial dysfunction in the aging process ( Dai et al., 2012 ; Park...

... . 10.1111/j.1439-0264.2005.00612.x Kim , M. J. , Kang , K. H. , Kim , C.-H. and Choi , S.-Y. ( 2008 ). Real-time imaging of mitochondria in transgenic zebrafish expressing mitochondrially targeted GFP . BioTechniques   45 , 331 - 334 . 10.2144/000112909 Kljavin , I. J...

... of the mitochondria, their morphology, and their respiration in human dermal fibroblasts. Here, we use this protocol to investigate applicability of power law diffusion to describe mitochondrial locomotion, assessment of rates of fission and fusion in healthy and diseased cells, and differences in mitochondria...

... Mitochondria Genomics Myogenesis Feed is the single largest cost in many intensive livestock farming enterprises including beef feedlotting ( Robinson and Oddy, 2004 ). Consequently, the food production industry is interested in enhancing the feed conversion efficiency of its major production species...

...) hardly induced stalk cell differentiation under the same conditions. Microscopic analyses revealed that the biologically active derivative, DIF-1-BODIPY, was incorporated by stalk cells at late stages of differentiation and was localized to mitochondria. The mitochondrial uncouplers carbonyl cyanide m...

...Susann Lehmann; Samantha H. Y. Loh; L. Miguel Martins ABSTRACT Familial forms of Parkinson's disease (PD) caused by mutations in PINK1 are linked to mitochondrial impairment. Defective mitochondria are also found in Drosophila models of PD with pink1 mutations. The co-enzyme nicotinamide adenine...

... - 309 . 10.1196/annals.1293.029 Barth , E. , Stammler , G. , Speiser , B. and Schaper , J. ( 1992 ). Ultrastructural quantitation of mitochondria and myofilaments in cardiac muscle from 10 different animal species including man . J. Mol. Cell. Cardiol.   24 , 669 - 681...

... Mitochondria Geometric morphometrics Livebearing freshwater fishes in the genus Limia (Poeciliidae) are diverse in their morphology, behavior, and ecology. In terms of overall species diversity and distribution, the genus Limia is one of the dominant freshwater fish groups in the West Indies...