... to produce the microtubules that form the mitotic spindle. Here, we identified phosphosites S11 and S36 in maternally originated Spc110 and explored their functions in vivo . Yeast expressing non-phosphorylatable Spc110 S11A had a distinct spindle phenotype characterised by higher levels of α-tubulin, which...

...Andrew Poulos; Breane G. Budaitis; Kristen J. Verhey ABSTRACT Kinesin motor proteins are responsible for orchestrating a variety of microtubule-based processes including intracellular transport, cell division, cytoskeletal organization, and cilium function. Members of the kinesin-6 family play...

...Hirohisa Ebina; Liang Ji; Masamitsu Sato ABSTRACT Microtubules in the mitotic spindle are organised by microtubule-associated proteins. In the late stage of mitosis, spindle microtubules are robustly organised through bundling by the antiparallel microtubule bundler Ase1/PRC1. In early mitosis...

...Mami Nomura; Kohei Atsuji; Keiko Hirose; Kogiku Shiba; Ryuji Yanase; Takeshi Nakayama; Ken-ichiro Ishida; Kazuo Inaba ABSTRACT A haptonema is an elongated microtubule-based motile organelle uniquely present in haptophytes. The most notable and rapid movement of a haptonema is ‘coiling’, which...

...Eric Denarier; Carine Brousse; Abdoulaye Sissoko; Annie Andrieux; Cécile Boscheron ABSTRACT Malformations of the human cerebral cortex can be caused by mutations in tubulins that associate to compose microtubules. Cerebral cortical folding relies on neuronal migration and on progenitor...

...Arthur T. Molines; Jessica Marion; Salem Chabout; Laetitia Besse; Jim P. Dompierre; Grégory Mouille; Frédéric M. Coquelle ABSTRACT Microtubules are involved in plant development and adaptation to their environment, but the sustaining molecular mechanisms remain elusive. Microtubule-end-binding 1...

...Nicole M. Myer; Kenneth A. Myers ABSTRACT Endothelial cell (EC) branching is critically dependent upon the dynamic nature of the microtubule (MT) cytoskeleton. Extracellular matrix (ECM) mechanosensing is a prominent mechanism by which cytoskeletal reorganization is achieved; yet how ECM-induced...

...Taylor A. Reid; Courtney Coombes; Melissa K. Gardner ABSTRACT Microtubules are structural polymers that participate in a wide range of cellular functions. The addition and loss of tubulin subunits allows the microtubule to grow and shorten, as well as to develop and repair defects and gaps in its...

...Jack W. C. Chen; Zhuo A. Chen; Kacper B. Rogala; Jeremy Metz; Charlotte M. Deane; Juri Rappsilber; James G. Wakefield ABSTRACT The hetero-octameric protein complex, Augmin, recruits γ-Tubulin ring complex (γ-TuRC) to pre-existing microtubules (MTs) to generate branched MTs during mitosis...

...Daniel G. Chawla; Ruchi V. Shah; Zachary K. Barth; Jessica D. Lee; Katherine E. Badecker; Anar Naik; Megan M. Brewster; Timothy P. Salmon; Nina Peel ABSTRACT Microtubule glutamylation is an important modulator of microtubule function and has been implicated in the regulation of centriole stability...

...Oliver Wueseke; David Zwicker; Anne Schwager; Yao Liang Wong; Karen Oegema; Frank Jülicher; Anthony A. Hyman; Jeffrey B. Woodruff ABSTRACT Centrosomes are major microtubule-organizing centers composed of centrioles surrounded by an extensive proteinacious layer called the pericentriolar material...

...Anna Melkov; Yasmin Simchoni; Yehonatan Alcalay; Uri Abdu ABSTRACT The microtubule (MT) plus-end motor kinesin heavy chain (Khc) is well known for its role in long distance cargo transport. Recent evidence showed that Khc is also required for the organization of the cellular MT network by mediating...

...Naoka Tamura; Judith E. Simon; Arnab Nayak; Rajesh Shenoy; Noriko Hiroi; Viviane Boilot; Akira Funahashi; Viji M. Draviam ABSTRACT Microtubules execute diverse mitotic events that are spatially and temporally separated; the underlying regulation is poorly understood. By combining drug treatments...

...Frédérique Carlier-Grynkorn; Liang Ji; Vincent Fraisier; Berangère Lombard; Florent Dingli; Damarys Loew; Anne Paoletti; Xavier Ronot; Phong T. Tran ABSTRACT The microtubule cytoskeleton plays important roles in cell polarity, motility and division. Microtubules inherently undergo dynamic...

...Régine Roubin; Claire Acquaviva; Véronique Chevrier; Fatima Sedjaï; Déborah Zyss; Daniel Birnbaum; Olivier Rosnet Summary The generation of cellular microtubules is initiated at specific sites such as the centrosome and the Golgi apparatus that contain nucleation complexes rich in γ-tubulin...