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Keywords: Mesenchymal stem cells
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Journal Articles

Osteogenic differentiation potential of porcine bone marrow mesenchymal stem cell subpopulations selected in different basal media

Sangeetha Kannan, Jyotirmoy Ghosh, Sujoy K. Dhara
Journal: Biology Open
Biol Open (2020) 9 (10): bio053280.
https://doi.org/10.1242/bio.053280
Published: 19 October 2020
...Sangeetha Kannan; Jyotirmoy Ghosh; Sujoy K. Dhara ABSTRACT Multipotent porcine mesenchymal stem cells (pMSC) are invaluable for research and therapeutic use in regenerative medicine. Media used for derivation and expansion of pMSC may play an important role for the selection of MSC subpopulation...
View articletitled, Osteogenic differentiation potential of porcine bone marrow mesenchymal stem cell subpopulations selected in different basal media
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Includes: Supplementary data
Journal Articles

Mechanical and molecular parameters that influence the tendon differentiation potential of C3H10T1/2 cells in 2D- and 3D-culture systems

Ludovic Gaut, Marie-Ange Bonnin, Cédrine Blavet, Isabelle Cacciapuoti, Monika Orpel, Mathias Mericskay, Delphine Duprez
Journal: Biology Open
Biol Open (2020) 9 (2): bio047928.
https://doi.org/10.1242/bio.047928
Published: 30 January 2020
... cell differentiation have not been identified. Mesenchymal stem cells present the ability to differentiate into multiple lineages in cultures under different cues ranging from chemical treatment to physical constraints. We analyzed the tendon differentiation potential of C3H10T1/2 cells, a murine cell...
View articletitled, Mechanical and molecular parameters that influence the tendon differentiation potential of C3H10T1/2 cells in 2D- and 3D-culture systems
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Journal Articles

A solution to prevent secondary flow in adherent cell cultures

Peter Szaraz, Matthew Librach, Poonam Mander, Banafshe Hoseini, Max Librach, Farwah Iqbal, Clifford Librach
Journal: Biology Open
Biol Open (2019) 8 (7): bio045294.
https://doi.org/10.1242/bio.045294
Published: 25 July 2019
... were augmented with a central column to diminish secondary flow. Human carcinoma cell lines (BeWo, JEG-3), mesenchymal stem cells [human umbilical cord perivascular cells (HUCPVC)] and mouse embryonic fibroblasts (MEF) were cultured in both column-augmented and regular culture wells. Human carcinoma...
View articletitled, A solution to prevent secondary flow in adherent cell cultures
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Includes: Supplementary data
Journal Articles

Soft substrate maintains proliferative and adipogenic differentiation potential of human mesenchymal stem cells on long-term expansion by delaying senescence

Sanjay Kumar Kureel, Pankaj Mogha, Akshada Khadpekar, Vardhman Kumar, Rohit Joshi, Siddhartha Das, Jayesh Bellare, Abhijit Majumder
Journal: Biology Open
Biol Open (2019) 8 (4): bio039453.
https://doi.org/10.1242/bio.039453
Published: 26 April 2019
...Sanjay Kumar Kureel; Pankaj Mogha; Akshada Khadpekar; Vardhman Kumar; Rohit Joshi; Siddhartha Das; Jayesh Bellare; Abhijit Majumder ABSTRACT Human mesenchymal stem cells (hMSCs), during in vitro expansion, gradually lose their distinct spindle morphology, self-renewal ability, multi-lineage...
View articletitled, Soft substrate maintains proliferative and adipogenic differentiation potential of human mesenchymal stem cells on long-term expansion by delaying senescence
Open the PDF for Soft substrate maintains proliferative and adipogenic differentiation potential of human mesenchymal stem cells on long-term expansion by delaying senescence in another window
Includes: Supplementary data