... disease. For detailed analysis, a useful model for fatty liver is needed and fish are considered as a potential candidate. We assessed through direct observation of the liver, which is the most conventional method for non-invasive analysis of progression in fatty liver. By using transparent medaka...

...Qizhi Liu; Yongming Yuan; Feng Zhu; Yunhan Hong; Ruowen Ge ABSTRACT Gene editing with CRISPR/Cas9 is a powerful tool to study the function of target genes. Although this technology has demonstrated wide efficiency in many species, including fertilized zebrafish and medaka fish embryos when...

.... Here, we developed a simple but sensitive method for quantifying the OMR using medaka ( Oryzias latipes ) as a model. This method, which simply requires video-recorded behavior, free tracking software, and a generic spreadsheet program, enables the evaluation of spectral sensitivity by researchers...

..., which suppresses gene flow between populations and may promote speciation. There are two strains of medaka ( Oryzias latipes ) that differ by a single transgene and mate assortatively depending on skin color. Here, we demonstrate that symmetrically biased (mutually exclusive) sexual preferences are (1...

... targeted mutagenesis using RGENs in medaka, Oryzias latipes . Somatic and heritable mutations were induced with high efficiency at the targeted genomic sequence on the DJ-1 gene in embryos that had been injected with the single guide RNA (sgRNA) transcribed by a T7 promoter and capped RNA encoding a Cas9...