... genetic and immunofluorescence microscopy toolkits, studies of Drosophila fat body function are ripe for cell biological analysis. Unlike the larval fat body, which is easily removed as a single, cohesive sheet of tissue, isolating intact adult fat body proves to be more challenging, thus hindering...

...) for 48 h. We used whole-mount immunofluorescent staining according to established protocols to detect various antigens (laminin, pan-cytokeratin, BrdU). In all cases, WT and transgenic embryos were euthanized by an overdose of 1% MS222, fixed in 4% PFA for 10 min at RT, and then overnight at 4°C...