... efficient automated cell counting workflows capable of quantifying cells in the developing wing. Our workflows can count the total number of cells or count cells in clones labeled with a fluorescent nuclear marker in imaginal discs. Moreover, by training a machine-learning algorithm we have developed...

... was carried out as described by de Celis and Bray ( de Celis and Bray, 1997 ). We analysed at least 10 wings from females of each genotype. Wing size, imaginal disc size, cell size, cell number data and ratios between number of cells were expressed as means ± standard error of the mean (SEM) and were...