...Tomohiro Tamari; Yoshihisa Ikeda; Kento Morimoto; Keiko Kobayashi; Saori Mizuno-Iijima; Shinya Ayabe; Akihiro Kuno; Seiya Mizuno; Atsushi Yoshiki ABSTRACT Genetically engineered mouse models are essential tools for understanding mammalian gene functions and disease pathogenesis. Genome editing...

... medium provided that the original work is properly attributed. Summary: Here, we describe and model a novel gene drive strategy – integral gene drive – for population replacement based on the hijacking of endogenous genes. Gene drive Population replacement CRISPR Genome editing...

... (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Summary: In this paper, we report an efficient genome editing method for cultured medaka fish cells using pre-formed...

...Samuel J. Capon; Gregory J. Baillie; Neil I. Bower; Jason A. da Silva; Scott Paterson; Benjamin M. Hogan; Cas Simons; Kelly A. Smith ABSTRACT The advent of genome editing has significantly altered genetic research, including research using the zebrafish model. To better understand the selectivity...

..., distribution and reproduction in any medium provided that the original work is properly attributed. Host-transfer TALEN Genome editing Targeted gene knockout Xenopus laevis Targeted gene disruption using either transcription activator-like effector nucleases (TALENs) (Christian et al., 2010...

...Satoshi Ansai; Masato Kinoshita ABSTRACT Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system-based RNA-guided endonuclease (RGEN) has recently emerged as a simple and efficient tool for targeted genome editing. In this study, we showed successful...

... and reproduction in any medium provided that the original work is properly attributed. Xenopus tropicalis TALENs Genome editing Targeted gene knockout Gene knockout is an important method used to demonstrate the function of a specific gene. Targeted gene disruption by homologous recombination...

... can be estimated from the numbers of blue and white colonies. Insertions and/or deletions were easily determined by sequencing the plasmid DNAs recovered from the positive colonies. Our technique should offer broad application to the artificial nucleases for genome editing in various types of model...