... of malaria transmission . Malar. J.   3 , 29 . 10.1186/1475-2875-3-29 Ding , D. , Chen , K. , Chen , Y. , Li , H. and Xie , K. ( 2018 ). Engineering introns to express RNA guides for Cas9- and Cpf1-mediated multiplex genome editing . Mol. Plant   11 , 542 - 552 . 10.1016...

... Attribution License ( http://creativecommons.org/licenses/by/3.0 ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Summary: In this paper, we report an efficient genome editing method for cultured medaka fish cells...

...Samuel J. Capon; Gregory J. Baillie; Neil I. Bower; Jason A. da Silva; Scott Paterson; Benjamin M. Hogan; Cas Simons; Kelly A. Smith ABSTRACT The advent of genome editing has significantly altered genetic research, including research using the zebrafish model. To better understand the selectivity...

..., distribution and reproduction in any medium provided that the original work is properly attributed. Host-transfer TALEN Genome editing Targeted gene knockout Xenopus laevis Targeted gene disruption using either transcription activator-like effector nucleases (TALENs) ( Christian et al...

...Satoshi Ansai; Masato Kinoshita ABSTRACT Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system-based RNA-guided endonuclease (RGEN) has recently emerged as a simple and efficient tool for targeted genome editing. In this study, we showed successful...

... for Scientific Research [25430089 to K.N.]. References Bedell V. M. , Wang Y. , Campbell J. M. , Poshusta T. L. , Starker C. G. , Krug R. G. I. I. , Tan W. , Penheiter S. G. , Ma A. C. , Leung A. Y. ( 2012 ). In vivo genome editing using a high...

... can be estimated from the numbers of blue and white colonies. Insertions and/or deletions were easily determined by sequencing the plasmid DNAs recovered from the positive colonies. Our technique should offer broad application to the artificial nucleases for genome editing in various types of model...