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Keywords: Gal4-UAS
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Journal Articles
Biol Open (2016) 5 (12): 1770–1775.
Published: 9 December 2016
... in diurnal environments. To generate transgenic lines that express α1ACT33Q through the Gal4-UAS system ( Brand and Perrimon, 1993 ), the DNA sequence covering exon 40 to the 3′ end of human CACNA1A was PCR-amplified using the primers 5′-ATGATCATGGAGTACTACCGGCAGA-3′ and 5′-TTAGCACCAATCATCGTCACTCTCG-3...