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Keywords: Electroporation
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Journal Articles
Tomohiro Tamari, Yoshihisa Ikeda, Kento Morimoto, Keiko Kobayashi, Saori Mizuno-Iijima, Shinya Ayabe, Akihiro Kuno, Seiya Mizuno, Atsushi Yoshiki
Journal:
Biology Open
Biol Open (2023) 12 (9): bio059970.
Published: 25 August 2023
... allows the generation of these models in multiple inbred strains of mice without backcrossing. Zygote electroporation dramatically removed the barrier for introducing the CRISPR-Cas9 complex in terms of cost and labour. Here, we demonstrate that the generalised zygote electroporation method is also...
Includes: Supplementary data
Journal Articles
Journal:
Biology Open
Biol Open (2019) 8 (4): bio039115.
Published: 26 April 2019
... in as close to an in vivo context as possible. Here we use electroporation of plasmid reporters to define critical parameters of a specific cis-regulatory element, ThrbCRM1, during retinal development. ThrbCRM1 is associated with cone photoreceptor genesis and activated in a subset of developing retinal cells...
Includes: Supplementary data
Journal Articles
Journal:
Biology Open
Biol Open (2018) 7 (8): bio035170.
Published: 10 August 2018
... haploid and diploid cells were transfected with the RNP complex by electroporation. Efficient gene editing was demonstrated by polymerase chain reaction (PCR) amplification of the target gene from genomic DNA and heteroduplex mobility assay carried out with polyacrylamide gel electrophoresis (PAGE...
Includes: Supplementary data