... health issues. Research on the underlying mechanisms, drug discovery, and the effects of aging and stress is limited. We used Drosophila , due to its similarity to the human renal system and ease of use, to identify cancer hallmarks and renal stone formation related to aging and oxidative stress. Our...

...Hye Jin Hwang; Rachel T. Cox ABSTRACT Drosophila oogenesis has long been an important model for understanding myriad cellular processes controlling development, RNA biology and patterning. Flies are easily fed drugs to disrupt various molecular pathways. However, this is often done under poor...

...Jun-yi Zhu; Joyce van de Leemput; Zhe Han ABSTRACT The multiprotein complexes known as the complex of proteins associated with Set1 (COMPASS) play a crucial role in the methylation of histone 3 lysine 4 (H3K4). In Drosophila , the COMPASS series complexes comprise core subunits Set1, Trx, and Trr...

...Rachael K. Ott; Isaiah H. Williams; Alissa R. Armstrong ABSTRACT Energy storage and endocrine functions of the Drosophila fat body make it an excellent model for elucidating mechanisms that underlie physiological and pathophysiological organismal metabolism. Combined with Drosophila's robust...

... such as Drosophila melanogaster , where it is relatively simple to obtain high sample numbers for downstream analyses. However, the developing wing, an intensively utilized structure in developmental biology, has eluded efficient cell counting workflows due to its highly dense cellular population. Here, we present...

... biosensors for apoptosis. Here we developed Necrosensor, a genetically encoded fluorescent sensor that detects necrosis in Drosophila , by utilizing HMGB1, which is released from the nucleus as a damage-associated molecular pattern (DAMP). We demonstrate that Necrosensor is able to detect necrosis induced...

...Zahraa A. Ghosn; Kailynn M. Sparks; Jacob L. Spaulding; Sanjana Vutukuri; Mirza J. J. Ahmed; Mark F. A. VanBerkum ABSTRACT Dysregulation of manganese (Mn) homeostasis is a contributing factor in many neuro-degenerative diseases. Adult Drosophila are sensitive to excessive levels of dietary Mn...

... these functions are becoming clear, less is known about how alterations in macrophage metabolism influence their roles as regulators of systemic physiology. Here, we investigate this by examining Drosophila macrophage-like cells called hemocytes. We used knockdown of TFAM, a mitochondrial genome transcription...

..., Drosophila melanogaster , genetically encoded metabolic FRET-based sensors that had been previously developed in single-cell systems. These sensors provide semi-quantitative data on the stationary concentrations of key metabolites of the bioenergetic metabolism: lactate, pyruvate, and 2-oxoglutarate. The use...

...Yan Wang; Roger Lopez-Bellido; Xiaojiao Huo; Annemieke Kavelaars; Michael J. Galko ABSTRACT Early phase diabetes is often accompanied by pain sensitization. In Drosophila , the insulin receptor ( InR ) regulates the persistence of injury-induced thermal nociceptive sensitization. Whether Drosophila...

... of neural circuits in both vertebrates and invertebrates, and may also contribute to the pathogenesis of neuropsychiatric disorders, such as autism and schizophrenia. In the peripheral nervous system (PNS), class IV dendritic arborization (da) sensory neurons of Drosophila , selectively remove the dendrites...

... show that a 24-amino-acid sequence from the Drosophila proapoptotic protein Hid's tail anchor (HTA) domain can target exogenous proteins to the mitochondria in Drosophila cells. We use this HTA tag to target the Drosophila centriole cartwheel protein Sas6 to the mitochondria, and show that both...

...Stephan U. Gerlach; Geert de Vreede; David Bilder ABSTRACT Animal organs maintain tissue integrity and ensure removal of aberrant cells through several types of surveillance mechanisms. One prominent example is the elimination of polarity-deficient mutant cells within developing Drosophila imaginal...

... tumor mutants in Drosophila by inducing antimicrobial peptides (AMP). Here, we investigated the regulatory mechanism underlying the activation in the mutant. Firstly, we found that reactive oxygen species (ROS) accumulated in the hemocytes due to induction of dual oxidase and one of its activators...

...Sophie L. Frampton; Catherine Sutcliffe; Clair Baldock; Hilary L. Ashe ABSTRACT A BMP gradient is essential for patterning the dorsal-ventral axis of invertebrate and vertebrate embryos. The extracellular BMP binding protein Short Gastrulation (Sog) in Drosophila plays a key role in BMP gradient...

... the pathway. However, visualising ERK activity in vivo with high temporal resolution remains a challenge in Drosophila . We adapted a kinase translocation reporter (KTR) for use in Drosophila , which shuttles out of the nucleus when phosphorylated by ERK. We show that ERK-KTR faithfully reports endogenous ERK...

... have revealed regulatory paradigms for stem cell self-renewal and differentiation. The germarium of the female Drosophila gonad, which houses both germline and somatic stem cells, is one such model system. Bulk mRNA sequencing (RNA-seq), single-cell RNA-seq (scRNA-seq), and bulk translation efficiency...

...Kerem Yildirim; Bente Winkler; Nicole Pogodalla; Steffi Mackensen; Marie Baldenius; Luis Garcia; Elke Naffin; Silke Rodrigues; Christian Klämbt ABSTRACT Neuronal processing is energy demanding and relies on sugar metabolism. To nurture the Drosophila nervous system, the blood-brain barrier forming...

... transduction, by promoting the phosphorylation of the Wnt co-receptor, LRP6, and the release of β-catenin from the adherens junctions. To assess the relevance of these previous findings in vivo , we analysed the consequence of the absence of Arf6 activity on Drosophila wing patterning, a developmental model...

..., we find a mutant, stum 9-3 , affecting the proliferation and differentiation of Drosophila adult ISCs in a forward genetic screen for factors regulating the proliferation and differentiation ISCs. stum 9-3 acts through the conserved Notch signaling pathway, upstream of the S2 cleavage of the Notch...