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Keywords: Drosophila
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Biol Open (2024) 13 (2): bio060254.
Published: 22 February 2024
... such as Drosophila melanogaster , where it is relatively simple to obtain high sample numbers for downstream analyses. However, the developing wing, an intensively utilized structure in developmental biology, has eluded efficient cell counting workflows due to its highly dense cellular population. Here, we present...
Includes: Supplementary data
Journal Articles
Biol Open (2024) 13 (1): bio060104.
Published: 22 January 2024
... biosensors for apoptosis. Here we developed Necrosensor, a genetically encoded fluorescent sensor that detects necrosis in Drosophila , by utilizing HMGB1, which is released from the nucleus as a damage-associated molecular pattern (DAMP). We demonstrate that Necrosensor is able to detect necrosis induced...
Includes: Supplementary data
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Biol Open (2024) 13 (1): bio060204.
Published: 5 January 2024
...Zahraa A. Ghosn; Kailynn M. Sparks; Jacob L. Spaulding; Sanjana Vutukuri; Mirza J. J. Ahmed; Mark F. A. VanBerkum ABSTRACT Dysregulation of manganese (Mn) homeostasis is a contributing factor in many neuro-degenerative diseases. Adult Drosophila are sensitive to excessive levels of dietary Mn...
Includes: Supplementary data
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Biol Open (2023) 12 (10): bio060030.
Published: 5 October 2023
..., Drosophila melanogaster , genetically encoded metabolic FRET-based sensors that had been previously developed in single-cell systems. These sensors provide semi-quantitative data on the stationary concentrations of key metabolites of the bioenergetic metabolism: lactate, pyruvate, and 2-oxoglutarate. The use...
Journal Articles
Biol Open (2023) 12 (6): bio059864.
Published: 1 June 2023
...Yan Wang; Roger Lopez-Bellido; Xiaojiao Huo; Annemieke Kavelaars; Michael J. Galko ABSTRACT Early phase diabetes is often accompanied by pain sensitization. In Drosophila , the insulin receptor ( InR ) regulates the persistence of injury-induced thermal nociceptive sensitization. Whether Drosophila...
Journal Articles
Biol Open (2023) 12 (1): bio059535.
Published: 6 January 2023
... of neural circuits in both vertebrates and invertebrates, and may also contribute to the pathogenesis of neuropsychiatric disorders, such as autism and schizophrenia. In the peripheral nervous system (PNS), class IV dendritic arborization (da) sensory neurons of Drosophila , selectively remove the dendrites...
Includes: Supplementary data
Journal Articles
Biol Open (2022) 11 (11): bio059545.
Published: 18 November 2022
... show that a 24-amino-acid sequence from the Drosophila proapoptotic protein Hid's tail anchor (HTA) domain can target exogenous proteins to the mitochondria in Drosophila cells. We use this HTA tag to target the Drosophila centriole cartwheel protein Sas6 to the mitochondria, and show that both...
Includes: Supplementary data
Journal Articles
Biol Open (2022) 11 (11): bio059525.
Published: 10 November 2022
...Stephan U. Gerlach; Geert de Vreede; David Bilder ABSTRACT Animal organs maintain tissue integrity and ensure removal of aberrant cells through several types of surveillance mechanisms. One prominent example is the elimination of polarity-deficient mutant cells within developing Drosophila imaginal...
Includes: Supplementary data
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Journal Articles
Biol Open (2022) 11 (6): bio059199.
Published: 10 June 2022
...Sophie L. Frampton; Catherine Sutcliffe; Clair Baldock; Hilary L. Ashe ABSTRACT A BMP gradient is essential for patterning the dorsal-ventral axis of invertebrate and vertebrate embryos. The extracellular BMP binding protein Short Gastrulation (Sog) in Drosophila plays a key role in BMP gradient...
Includes: Supplementary data
Journal Articles
Biol Open (2022) 11 (5): bio059364.
Published: 24 May 2022
... the pathway. However, visualising ERK activity in vivo with high temporal resolution remains a challenge in Drosophila . We adapted a kinase translocation reporter (KTR) for use in Drosophila , which shuttles out of the nucleus when phosphorylated by ERK. We show that ERK-KTR faithfully reports endogenous ERK...
Includes: Supplementary data
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Journal Articles
Biol Open (2022) 11 (1): bio059128.
Published: 18 January 2022
...Kerem Yildirim; Bente Winkler; Nicole Pogodalla; Steffi Mackensen; Marie Baldenius; Luis Garcia; Elke Naffin; Silke Rodrigues; Christian Klämbt ABSTRACT Neuronal processing is energy demanding and relies on sugar metabolism. To nurture the Drosophila nervous system, the blood-brain barrier forming...
Includes: Supplementary data
Journal Articles
Biol Open (2021) 10 (12): bio058892.
Published: 2 December 2021
... transduction, by promoting the phosphorylation of the Wnt co-receptor, LRP6, and the release of β-catenin from the adherens junctions. To assess the relevance of these previous findings in vivo , we analysed the consequence of the absence of Arf6 activity on Drosophila wing patterning, a developmental model...
Includes: Supplementary data
Journal Articles
Biol Open (2021) 10 (11): bio058910.
Published: 3 November 2021
..., we find a mutant, stum 9-3 , affecting the proliferation and differentiation of Drosophila adult ISCs in a forward genetic screen for factors regulating the proliferation and differentiation ISCs. stum 9-3 acts through the conserved Notch signaling pathway, upstream of the S2 cleavage of the Notch...
Includes: Supplementary data
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Biol Open (2021) 10 (8): bio058722.
Published: 17 August 2021
..., phylogenetically distant counterpart – the male accessory gland (MAG) – in insects like Drosophila . It has not been ascertained if the anatomically disparate Drosophila MAG shares developmental parallels with those of the mammalian prostate. Here we show that the development of Drosophila mesoderm-derived MAG...