... to initiation of co-culture experiments. Hepatocyte aggregates were dissociated with 0.5% Trypsin-EDTA for 7 min at 37°C. At the same time, macrophages, MSCs, and endothelial cells were dissociated with TrypLE Select for 5–7 min at 37°C. All cells were then suspended to a density of 1×10 6 cells/ml...

... and absence of stress hormones. In this report, effects of cortisol and serotonin on the kinetics of immune and metabolic changes in adipocytes and macrophage (alone and co-cultured) was studied through whole genome transcriptome profiling. A transition from pro- to anti-inflammatory response in the immune...

...Yu Nan Jiang; Jun Zhao; Feng Ting Chu; Yang Yang Jiang; Guo Hua Tang ABSTRACT Co-culture of bone marrow stromal cells (BMSCs) and vascular endothelial cells (VECs) is a promising strategy for better osteogenesis and pre-vascularization in bone tissue engineering. Recent reports have shown...

...Haiyan Ding; Zhihui Cheng; Menglong Liu; Sikandar Hayat; Han Feng ABSTRACT A hydroponic co-culture system was adopted to determine the allelopathic potential of garlic on the growth of pepper plants. Different numbers of garlic plants (0, 2, 4, 8 and 12) were hydroponically co-cultured with two...