... show that a 24-amino-acid sequence from the Drosophila proapoptotic protein Hid's tail anchor (HTA) domain can target exogenous proteins to the mitochondria in Drosophila cells. We use this HTA tag to target the Drosophila centriole cartwheel protein Sas6 to the mitochondria, and show that both...

...Shohei Yamamoto; Ryoichi Yabuki; Daiju Kitagawa ABSTRACT The deuterosome is a non-membranous organelle involved in large-scale centriole amplification during multiciliogenesis. Deuterosomes are specifically assembled during the process of multiciliogenesis. However, the molecular mechanisms...

...Rustem Uzbekov; Anastasiia Garanina; Christophe Bressac ABSTRACT The centrosome is the organizing center of microtubules in the cell, the basis for the origin of cilia and flagella and a site for the concentration of a regulatory proteins multitude. The centrosome comprises two centrioles...

.... Tetrahymena Basal body Centriole Cilia Polarity Poc1 Automated image analysis Tetrahymena thermophila is a unicellular ciliate that employs multi-ciliary arrays for propulsion in an aqueous environment ( Frankel, 2000 ; Hennessey and Lampert, 2012 ). A single Tetrahymena cell contains...

...Jennifer H. Richens; Teresa P. Barros; Eliana P. Lucas; Nina Peel; David Miguel Susano Pinto; Alan Wainman; Jordan W. Raff ABSTRACT Centrosomes comprise a pair of centrioles surrounded by a matrix of pericentriolar material (PCM). In vertebrate cells, Pericentrin plays an important part in mitotic...

... that typically grows from the mother centriole in G0/G1 phase of the cell cycle as a membrane-bound compartment that protrudes from the cell surface. A unique system of bidirectional transport, intraflagellar transport (IFT), maintains the structure and function of cilia. While the axoneme is dynamic, growing...

... to current experimental systems available. To overcome these limitations we have developed six new Drosophila cell lines derived from Drosophila homozygous mutants for DSas-4, a protein essential for centriole biogenesis. These cells lack detectable centrosomal structures, astral MT, with dispersed...

...Toshiyuki Hatano; Greenfield Sluder Summary Mother–daughter centriole disengagement, the necessary first step in centriole duplication, involves Plk1 activity in early mitosis and separase activity after APC/C activity mediates securin degradation. Plk1 activity is thought to be essential...

...Katharina F. Sonnen; Lothar Schermelleh; Heinrich Leonhardt; Erich A. Nigg Summary Centrioles are essential for the formation of cilia and flagella. They also form the core of the centrosome, which organizes microtubule arrays important for cell shape, polarity, motility and division. Here, we have...

...Kuo-Sheng Wu; Tang K. Tang Summary The primary cilium is a microtubule-based structure protruded from the basal body analogous to the centriole. CPAP (centrosomal P4.1-associated protein) has previously been reported to be a cell cycle-regulated protein that controls centriole length. Mutations...