... of the stem cell pool is associated with a decreased expression of the cell cycle inhibitor p21. Our findings show that the genetic background of a patient can directly alter stem cell function, provides new insights on the innate cellular dysregulation in MS and identifies p21 pathway as a new potential...

... trajectory of the brain is poorly understood and debated. Using long-term single-cell imaging, we show that disruption of a single copy of CHD8 in human neural precursor cells (NPCs) markedly shortens the G1 phase of the cell cycle. Consistent with faster progression of CHD8 +/− NPCs through G1 and the G1/S...

... analyzed by western blot. The results showed that exposure to ELF-MF did not induce DNA damage, changes to cell cycle distribution or increased reactive oxygen species level. No significant differences were detected in p53 and Hsp70 expression level between the ELF-MF and sham-exposure groups both in vitro...

... analysis using tandem mass spectrometry of enriched Saccharomyces cerevisiae SPBs for two cell cycle arrests, G1/S and the mitotic checkpoint, expanding on previously reported phosphoproteomic data sets. We present a novel phosphoproteomic state of SPBs arrested in G1/S by a cdc4-1 temperature-sensitive...

... of these events. Furthermore, it is thought that T. brucei lacks a spindle checkpoint that delays the onset of nuclear division in response to spindle defects. Here we show that a mitotic cyclin CYC6 has a dynamic localization pattern during the cell cycle, including kinetochore localization. Using CYC6...

...Aya Noguchi; Shungo Adachi; Naoto Yokota; Tomohisa Hatta; Tohru Natsume; Hiroyuki Kawahara ABSTRACT ZFP36L2 promotes the destruction of AU-rich element-containing transcripts, while its regulation and functional significance in cell cycle control are scarcely identified. We show that ZFP36L2...

... and is required for checkpoint-mediated cell cycle arrest . J. Cell Sci.   115 , 4555 - 4564 . 10.1242/jcs.00133 Carr , A. M. , Moudjou , M. , Bentley , N. J. and Hagan , I. M. ( 1995 ). The chk1 pathway is required to prevent mitosis following cell-cycle arrest at “start.” . Curr...

...-associated (Ska) complex regulates chromosome movement and the metaphase-to-anaphase cell cycle transition. This article has an associated First Person interview with the first author of the paper as part of the supplementary information. Mitosis Spindle checkpoint Cell cycle Microtubules Anaphase...

..., but on the basal side of the meristem cells exit the meristem through the transition zone and enter in the elongation zone, where they stop division and rapidly elongate. Throughout this journey cells are accompanied by changes in cell cycle progression. Flow cytometry analysis showed that meristematic cells...

... also leads to a cell cycle arrest at G1 phase. These regulatory roles of Akt3 are dependent on its kinase activity. Blocking the expression of Akt1 plus Akt2 in ESCs does not affect cell survival or proliferation, although blocking Akt1 aggravates the apoptotic effect induced by depletion of Akt3. We...

..., understanding the links between steroid hormone signaling and cell proliferation is crucial to understanding the molecular basis of morphogenesis. Here we examined the mechanism by which the steroid hormone ecdysone regulates the cell cycle in Drosophila . We find that a cell cycle arrest induced by ecdysone...

... of miR-93 promoted the proliferation, migration and invasion of glioma cells, and that miR-93 was involved in the regulation of cell cycle progression by mediating the protein levels of P21, P27, P53 and Cyclin D1. P21 was further identified as a direct target of miR-93. Knockdown of P21 attenuated...

... S-phase labelling to characterise changes in their cell cycle length. Our study revealed the persistence of Sox3-positive progenitor cells from the earliest stages of neural development through to the juvenile adult. Two periods of intense neuronal generation were observed, confirming the existence...

... of cell cycle, associated with increased levels of p53 protein and p53 target genes. G1 prolongation in ESCs was not associated with induction of differentiation. Loss of entry into S phase of the cell cycle and marked induction of nuclear p53 were also observed in PDCD2 knockout blastocysts...

... for correspondence ( [email protected] ) Competing interests The authors have no competing interests to declare. 11 11 2013 23 01 2014 Drosophila Ras Tumor Cell cycle Checkpoints Aneuploidy Traditional cancer therapy is tailored to the site of disease (ovary, lung, brain...

..., defining CS as a transcription syndrome is inconclusive. In particular, the function of XPG in transcription has not been clearly demonstrated. Here, we provide evidence for the involvement of RAD2 , the Saccharomyces cerevisiae counterpart of XPG , in cell cycle regulation and efficient actin assembly...

... compact zone myocardial cells, establishing a transmural cell cycle gradient. The molecular determinants of VCS/trabecular myocyte cell cycle arrest are not known. Given the importance of pocket proteins (Rb, p107 and p130) in mediating G0/G1 arrest in many cell types, we examined the role of this gene...

...Chloe Tuck; Tongli Zhang; Tamara Potapova; Marcos Malumbres; Béla Novák Summary Cell cycle events are driven by Cyclin dependent kinases (CDKs) and by their counter-acting phosphatases. Activation of the Cdk1:Cyclin B complex during mitotic entry is controlled by the Wee1/Myt1 inhibitory kinases...

... PSCs (rbiPSCs) that were obtained by reprogramming adult skin fibroblasts. All cell lines required fibroblast growth factor 2 for their growth and proliferation. All rbESC lines showed molecular and functional properties typically associated with primed pluripotency. The cell cycle of rbESCs had...

...Danming Tang; Hebao Yuan; Ole Vielemeyer; Franck Perez; Yanzhuang Wang Summary GRASP65 phosphorylation during mitosis and dephosphorylation after mitosis are required for Golgi disassembly and reassembly during the cell cycle. At least eight phosphorylation sites on GRASP65 have been identified...