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Keywords: CRISPR
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Journal Articles
Series: REVIEW COMMONS TRANSFER
Biol Open (2024) 13 (4): bio060401.
Published: 28 March 2024
... of the CRISPR/Cas method a decade ago. The generation of loss-of-function alleles via the production of small insertions or deletions in the coding sequences of genes with CRISPR/Cas systems is now routinely achieved with high efficiency. The method is based on the error prone repair of precisely targeted DNA...
Includes: Supplementary data
Journal Articles
Biol Open (2023) 12 (10): bio060122.
Published: 19 October 2023
...Mohamed Jemaà ABSTRACT Clustered regularly interspaced short palindromic repeats, or CRISPR, is a powerful molecular biology tool that is enabling high-quality genetic research and engineering. However, for practical reasons, but more specifically because of the lack of training and the rapid...
Journal Articles
Biol Open (2023) 12 (8): bio059994.
Published: 15 August 2023
...Laura Schubert; Anh T. Le; Trista K. Hinz; Andre C. Navarro; Sarah K. Nelson-Taylor; Raphael A. Nemenoff; Lynn E. Heasley; Robert C. Doebele ABSTRACT CRISPR/Cas9 gene editing represents a powerful tool for investigating fusion oncogenes in cancer biology. Successful experiments require that sgRNAs...
Includes: Supplementary data
Journal Articles
Biol Open (2023) 12 (3): bio059647.
Published: 21 March 2023
... and their function has been linked to neurodevelopmental disorders, cancer, and viral infections. Here, we report two new genome-edited mouse models, generated using a CRISPR-Cas9 approach, in which the amino acid residue lysine 50 is replaced with arginine 50 (K50R) in eIF5A1 or in the closely related eIF5A2...
Includes: Supplementary data
Journal Articles
Biol Open (2022) 11 (12): bio059695.
Published: 19 December 2022
... and incompletely understood. To shed light on this mystery, we performed a genome-wide CRISPR-Cas9 screen to systematically identify regulators of cancer resistance to the soluble short chain ceramide, C6 ceramide (C6-Cer). Our results reveal a complex landscape of genetic modifiers of C6-Cer toxicity, including...
Includes: Supplementary data
Journal Articles
Biol Open (2019) 8 (1): bio039552.
Published: 17 January 2019
... homologue of espin , to develop a unique genetic reporter for asymmetric oocyte organization. We generated a null allele of the forked gene using the CRISPR-Cas9 system and found that forked is not required for determining the axes of the Drosophila embryo. However, ectopic expression of a truncated form...
Includes: Supplementary data
Journal Articles
Biol Open (2019) 8 (1): bio037762.
Published: 3 January 2019
...Alexander Nash; Giulia Mignini Urdaneta; Andrea K. Beaghton; Astrid Hoermann; Philippos Aris Papathanos; George K. Christophides; Nikolai Windbichler ABSTRACT A first generation of CRISPR-based gene drives has now been tested in the laboratory in a number of organisms, including malaria vector...
Includes: Supplementary data
Journal Articles
Biol Open (2016) 5 (1): 83–89.
Published: 23 December 2015
... specific manner. T-STEP uses a combination of efficient CRISPR/Cas9-enhanced gene targeting and tissue-specific recombinase-mediated tag swapping to temporally and spatially label endogenous proteins. We have employed this method to GFP tag OCRL (a phosphoinositide-5-phosphatase in the endocytic pathway...
Includes: Supplementary data
Journal Articles
Biol Open (2014) 3 (1): 42–49.
Published: 6 December 2013
...Andrew R. Bassett; Charlotte Tibbit; Chris P. Ponting; Ji-Long Liu Summary We have applied the CRISPR/Cas9 system to Drosophila S2 cells to generate targeted genetic mutations in more than 85% of alleles. By targeting a constitutive exon of the AGO1 gene, we demonstrate homozygous mutation in up...
Includes: Supplementary data