... a novel Zic3 ins5V mouse line generated due to a mis-insertion during the CRISPR genome editing process, which altered the Zic3 5′UTR structure. Mice with this insertion developed similar phenotypes to Zic3 LacZ null mice, including heterotaxy, isolated heart defects, neural tube defects and tail kinks...

...Junyan Ma; Weiting Zhang; Simin Rahimialiabadi; Nikkitha Umesh Ganesh; Zhengwang Sun; Saba Parvez; Randall T. Peterson; Jing-Ruey Joanna Yeh ABSTRACT Here, we introduce ‘TICIT’, targeted integration by CRISPR-Cas9 and integrase technologies, which utilizes the site-specific DNA recombinase – phiC31...

... of the CRISPR/Cas method a decade ago. The generation of loss-of-function alleles via the production of small insertions or deletions in the coding sequences of genes with CRISPR/Cas systems is now routinely achieved with high efficiency. The method is based on the error prone repair of precisely targeted DNA...

...Mohamed Jemaà ABSTRACT Clustered regularly interspaced short palindromic repeats, or CRISPR, is a powerful molecular biology tool that is enabling high-quality genetic research and engineering. However, for practical reasons, but more specifically because of the lack of training and the rapid...

...Laura Schubert; Anh T. Le; Trista K. Hinz; Andre C. Navarro; Sarah K. Nelson-Taylor; Raphael A. Nemenoff; Lynn E. Heasley; Robert C. Doebele ABSTRACT CRISPR/Cas9 gene editing represents a powerful tool for investigating fusion oncogenes in cancer biology. Successful experiments require that sgRNAs...

... and their function has been linked to neurodevelopmental disorders, cancer, and viral infections. Here, we report two new genome-edited mouse models, generated using a CRISPR-Cas9 approach, in which the amino acid residue lysine 50 is replaced with arginine 50 (K50R) in eIF5A1 or in the closely related eIF5A2...

... and incompletely understood. To shed light on this mystery, we performed a genome-wide CRISPR-Cas9 screen to systematically identify regulators of cancer resistance to the soluble short chain ceramide, C6 ceramide (C6-Cer). Our results reveal a complex landscape of genetic modifiers of C6-Cer toxicity, including...

... homologue of espin , to develop a unique genetic reporter for asymmetric oocyte organization. We generated a null allele of the forked gene using the CRISPR-Cas9 system and found that forked is not required for determining the axes of the Drosophila embryo. However, ectopic expression of a truncated form...

...Alexander Nash; Giulia Mignini Urdaneta; Andrea K. Beaghton; Astrid Hoermann; Philippos Aris Papathanos; George K. Christophides; Nikolai Windbichler ABSTRACT A first generation of CRISPR-based gene drives has now been tested in the laboratory in a number of organisms, including malaria vector...

... specific manner. T-STEP uses a combination of efficient CRISPR/Cas9-enhanced gene targeting and tissue-specific recombinase-mediated tag swapping to temporally and spatially label endogenous proteins. We have employed this method to GFP tag OCRL (a phosphoinositide-5-phosphatase in the endocytic pathway...

...Andrew R. Bassett; Charlotte Tibbit; Chris P. Ponting; Ji-Long Liu Summary We have applied the CRISPR/Cas9 system to Drosophila S2 cells to generate targeted genetic mutations in more than 85% of alleles. By targeting a constitutive exon of the AGO1 gene, we demonstrate homozygous mutation in up...