... on various physiological parameters with the auxin-inducible degron system that permitted depletion of INTS-4 (Integrator subunit) catalytic subunit of the protein complex. We found that Integrator activity is critical and required for C. elegans development within the L1 larval stage, but becomes...

...Heino J. Hulsey-Vincent; Elizabeth A. Cameron; Caroline L. Dahlberg; Domenico F. Galati ABSTRACT Caenorhabditis elegans gut and cuticle produce a disruptive amount of autofluorescence during imaging. Although C. elegans autofluorescence has been characterized, it has not been characterized at high...

... proliferation ( Kwon et al., 2014 , 2016 ; Valdez et al., 2012 ). Based on ChIP-seq data, there are putative EGL-43 binding sites in the lin-12 locus ( Deng et al., 2020 ). The emergence of CRISPR/Cas9 as a gene-editing tool in C. elegans ( Vicencio and Cerón, 2021 ) should facilitate the modification...

..., at the same time, preventing spurious activation of deleterious genes. All experiments were performed on C. elegans hermaphrodites. Genotyping primers for ngn-1 , hlh-3 , hlh-16 , hlh-2 , egl-1 , rrf-3 and ttx-3 mutants are presented in Table S2 . The ttx-3endo::yfp (vba3) and ceh...

..., but their morphology and location are consistent with endosomes described at C. elegans NMJs: They are roughly spherical and located within the synaptic vesicle pool ( Watanabe et al., 2013a ), and they do not resemble other intracellular structures, such as autophagosomes ( Meléndez et al., 2003...

...Maria Gravato-Nobre; Jonathan Hodgkin; Petros Ligoxygakis ABSTRACT The nematode worm Caenorhabditis elegans depends on microbes in decaying vegetation as its food source. To survive in an environment rich in opportunistic pathogens, C . elegans has evolved an epithelial defence system where surface...

..., aggregates of PQN-75 are occasionally found throughout the pharynx, suggesting secretion from pharyngeal gland cells into the surrounding pharyngeal muscle. PQN-75 does not affect fertility and brood size in C. elegans but confers some degree of stress resistance and thermotolerance through unknown...

... and temporally regulate glutamylation remains relatively unexplored. We have undertaken an analysis of the glutamylating TTLL enzymes in C. elegans . We find that although all five TTLL enzymes are expressed in the embryo and adult worm, loss of individual enzymes does not perturb microtubule function...

... that the original work is properly attributed. Summary: Polo-like kinase phosphorylation determines proper centrosome scaffold size and density, but not function or maintenance, in Caenorhabditis elegans embryos. C. elegans PCM Polo-like kinase SPD-5 Centrosome Microtubule...

... for studying gene expression in Caenorhabditis elegans . Gene 93 , 189 - 198 . 10.1016/0378-1119(90)90224-F Fritz , J.-A. and Behm , C. A. ( 2009 ). CUTI-1: a novel tetraspan protein involved in C. elegans cuticle formation and epithelial integrity . PLoS ONE 4 , e5117 . 10.1371...

...Tong Young Lee; Kyoung-hye Yoon; Jin Il Lee ABSTRACT The nematode Caenorhabditis elegans is one of the premier experimental model organisms today. In the laboratory, they display characteristic development, fertility, and behaviors in a two dimensional habitat. In nature, however, C. elegans...

... in glycopeptidolipid production is deficient in binding to human epithelial cells (HEp-2), as well as deficient in its ability to bind to and colonize the intestinal tract of C. elegans as efficiently as wild-type MAH. These data indicate the C. elegans may serve as a useful model system for MAH pathogenesis...

...://creativecommons.org/licenses/by/3.0 ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. C. elegans EIF4E Germline Sex-determination Spermatogenesis Oogenesis Eukaryotic initiation factor 4-complex (eIF4) recruits...

...Selma Waaijers; João Jacob Ramalho; Thijs Koorman; Elisabeth Kruse; Mike Boxem ABSTRACT Crumbs proteins are important regulators of epithelial polarity. In C. elegans , no essential role for the two described Crumbs homologs has been uncovered. Here, we identify and characterize an additional...

... provided that the original work is properly attributed. C. elegans molting larvae amyloid cuticle ABU/PQN innate immunity unfolded protein response Ecdysozoa, the clade of animals defined by the presence of an exoskeleton, exchange their cuticle during episodic molts ( Aguinaldo et...

... – 2229 . 10.1128/AAC.01097-06 Breger J. , Fuchs B. B. , Aperis G. , Moy T. I. , Ausubel F. M. , Mylonakis E. ( 2007 ). Antifungal chemical compounds identified using a C. elegans pathogenicity assay. PLoS Pathog. 3 , e18 . 10.1371/journal.ppat.0030018...

..., and apoptosis. Cooperative interactions between histone modifications such as acetylation, methylation and sumoylation are crucial for proper attenuation in C. elegans , implying that the proteins recognising these histone modifications could also play an important role in attenuation of RAS-mediated signalling...

.... , Zhang Y. , Herman M. A. , Okano H. , Sawa H. ( 2006 ). Wnt signaling and a Hox protein cooperatively regulate psa-3/Meis to determine daughter cell fate after asymmetric cell division in C. elegans. Dev. Cell 11 , 105 – 115 . 10.1016/j.devcel.2006.04.020 Blyth K...

... Commons Attribution Non-Commercial Share Alike License ( http://creativecommons.org/licenses/by-nc-sa/3.0/ ). C. elegans Centrosome Microtubules Mitotic spindle assembly PP2A Protein phosphatase Proteomics studies suggest that changes in protein phosphorylation are particularly...