... that are ubiquitous. Here we focus on actin cytoskeleton dynamics to explore the role of latrophilins in mammalian cells. Individual overexpression of each latrophilin isoform comparably increased cell volume while modifying the net profile of F-actin-dependent cell extensions, as evaluated by confocal microscopy...

...Daniela Leyton-Puig; Katarzyna M. Kedziora; Tadamoto Isogai; Bram van den Broek; Kees Jalink; Metello Innocenti ABSTRACT Super-resolution microscopy (SRM) allows precise localization of proteins in cellular organelles and structures, including the actin cytoskeleton. Yet sample preparation...

... through the binding to Src as well as phosphorylation-dependent association with actin retrograde flow at focal adhesions. However, it remains elusive how phosphorylated Cas interacts with actin cytoskeletons. We observe that the actin-binding protein, tensin 1, co-localizes with Cas, but not with its...

... divide. Of general significance, LifeAct-GFP negatively affects the actin cytoskeleton, indicating caution in its use as a biomarker for actin filaments. Thermal stability was measured by following the ellipticity of AS-Cdc8p (0.2 mg/ml) at 222 nm in 0.5 M NaCl, 10 mM sodium phosphate pH 7.5, 1 mM...

... MPO to platelets did not activate their aggregation under basal conditions (without agonist). In contrast, MPO augmented agonist-induced platelet aggregation, which was not prevented by MPO enzymatic activity inhibitors. It was found that exposure of platelets to MPO leads to actin cytoskeleton...

... on a synthetic matrix mimicking in vivo microenvironment. Importantly, we demonstrated that the organization of the F-actin network and acetylated microtubules of the cells were modified during the differentiation process. We showed that the perturbation of the F-actin cytoskeleton organization abolished...

...Naofumi Yui; Hua Jenny Lu; Richard Bouley; Dennis Brown Summary Remodeling of the actin cytoskeleton is required for vasopressin (VP)‐induced aquaporin 2 (AQP2) trafficking. Here, we asked whether VP and forskolin (FK)‐mediated F‐actin depolymerization depends on AQP2 expression. Using various MDCK...