...Laura A. Sherer; Biswaprakash Mahanta; Naomi Courtemanche ABSTRACT The actin cytoskeleton is a dynamic filamentous network that assembles into specialized structures to enable cells to perform essential processes. Direct visualization of fluorescently-labeled cytoskeletal proteins has provided...

...Nidhi Yadunandanan Nair; Victor Samuel; Lariza Ramesh; Areeba Marib; Deena T. David; Ananthalakshmy Sundararaman ABSTRACT Actin, one of the most abundant intracellular proteins in mammalian cells, is a critical regulator of cell shape and polarity, migration, cell division, and transcriptional...

...Ottilie von Loeffelholz; Andrew Purkiss; Luyan Cao; Svend Kjaer; Naoko Kogata; Guillaume Romet-Lemonne; Michael Way; Carolyn A. Moores ABSTRACT The Arp2/3 complex regulates many cellular processes by stimulating formation of branched actin filament networks. Because three of its seven subunits...

...Oliver Glomb; Lara Bareis; Nils Johnsson ABSTRACT The polarisome comprises a network of proteins that organizes polar growth in yeast and filamentous fungi. The yeast formin Bni1 and the actin nucleation-promoting factor Bud6 are subunits of the polarisome that together catalyze the formation...

...Lydie Couturier; Khalil Mazouni; Fred Bernard; Charlotte Besson; Elodie Reynaud; François Schweisguth ABSTRACT In epithelia, mitotic cells round up and push against their neighbors to divide. Mitotic rounding results from increased assembly of F-actin and cortical recruitment of Myosin II, leading...

... of osteoclasts derived from RAW264.7 cells transfected with EGFP-actin revealed consistent symmetrical retrograde actin flow in the ZLS, but not in the podosome cluster, the podosome ring or the podosome belt. Confocal imaging showed that the distributions of F-actin, vinculin, paxillin and zyxin in the ZLS were...

... droplets are reservoirs of neutral lipids that act as important cellular energy resources. Using live-cell imaging analysis, we found that the lipid droplets of mother cells redistribute to their nascent spores. Notably, this process was actin polymerization-dependent and facilitated by the leading edge...

..., mediating endocytosis of PM components. Dynamin redistributed off ingressed PM furrows in metaphase, correlating with stabilized PM components and the associated actin regulatory machinery on long furrows. Acute inhibition of dynamin in the temperature sensitive shibire mutant embryo resulted...

...Shigehiko Yumura; Sayaka Hashima; Satsuki Muranaka ABSTRACT Cells are always subjected to mechanical stresses, resulting in wounds of the cell membrane, but cells are able to repair and reseal their wounded membrane. Previous reports have shown that actin and myosin II accumulate around the wound...

..., Gobionotothen gibberifrons (Lönnberg) (habitat/body T = −1.9 to +2°C), and of the cow (body T = 37°C). We examined the temperature dependence of the binding of denatured CPs (β-actin, β-tubulin) by fish and bovine CCTs, both in homologous and heterologous combinations and at temperatures between −4°C and 20°C...

...Shigehiko Yumura; Go Itoh; Yumi Kikuta; Takeomi Kikuchi; Toshiko Kitanishi-Yumura; Masatsune Tsujioka Summary Actin and myosin II play major roles in cell migration. Whereas pseudopod extension by actin polymerization has been intensively researched, less attention has been paid to how the rest...

...J'Nelle S. Young; Marc De Asis; Julian Guttman; A. Wayne Vogl Summary Tubulobulbar complexes are actin-related endocytic structures that form at sites of intercellular attachment in the seminiferous epithelium and are proposed to internalize intact junctions. In this study, we test the prediction...

... of benign tumors, predominantly schwannomas, in the peripheral nervous system. Merlin links plasma membrane receptors with the actin cytoskeleton and its targeting to the plasma membrane depends on direct binding to the paxillin scaffold protein. Exon 2 of NF2 , an exon mutated in NF2 patients and deleted...

...Meikun Kan-O; Ryu Takeya; Takaya Abe; Naoyuki Kitajima; Motohiro Nishida; Ryuji Tominaga; Hitoshi Kurose; Hideki Sumimoto Summary Heart development requires organized integration of actin filaments into the sarcomere, the contractile unit of myofibrils, although it remains largely unknown how actin...